Omurtag G Z, Beyoğlu D
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Marmara University, 81010, Haydarpasa, Istanbul, Turkey.
Food Addit Contam. 2003 Apr;20(4):405-9. doi: 10.1080/0265203031000082512.
The aim was to investigate the occurrence of deoxynivalenol (DON) in cereal and pulse products in Turkey. DON was detected using high-performance liquid chromatography (HPLC) with ultraviolet detection at 220 nm and positive results greater or equal to 0.60 ppm were confirmed by thin layer chromatography (TLC). An acetonitrile-water (21:4 v/v) extract of the sample was cleaned up on a column packed with alumina-Celite-charcoal (0.35 + 0.25 + 0.40 g). The detection limits for DON were 3 ng/injection (0.10 ppm) and 50 ng/spot (0.60 ppm) for HPLC and TLC, respectively. Eighty-three commercially available cereal and pulse product samples collected from markets and street bazaars were analysed. The recovery rates for boiled, pounded wheat and rice spiked with added DON (1 ppm) were 80.9% (SD 8.37, n=5) and 72.3% (3.85, n=5), respectively. DON was detected in six (8.82%) of 68 cereal and in none of 15 pulse products. The maximum detected amount was 2.67 ppm in a corn flour sample.
目的是调查土耳其谷物和豆类产品中脱氧雪腐镰刀菌烯醇（DON）的出现情况。使用配备紫外检测器（检测波长为220 nm）的高效液相色谱法（HPLC）检测DON，阳性结果（大于或等于0.60 ppm）通过薄层色谱法（TLC）进行确认。将样品的乙腈 - 水（21:4 v/v）提取物在填充有氧化铝 - 硅藻土 - 活性炭（0.35 + 0.25 + 0.40 g）的柱上进行净化处理。HPLC和TLC对DON的检测限分别为3 ng/进样量（0.10 ppm）和50 ng/斑点（0.60 ppm）。对从市场和街头集市收集的83个市售谷物和豆类产品样品进行了分析。添加了DON（1 ppm）的煮制、研磨小麦和大米的回收率分别为80.9%（标准差8.37，n = 5）和72.3%（3.85，n = 5）。在68种谷物中的6种（8.82%）检测到了DON，而在15种豆类产品中均未检测到。在一个玉米粉样品中检测到的最大含量为2.67 ppm。
