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Crystallization and preliminary X-ray diffraction analysis of Jun a 1, the major allergen isolated from pollen of the mountain cedar Juniperus ashei.

作者信息

Liu Dequin, Midoro-Horiuti Terumi, White Mark A, Brooks Edward G, Goldblum Randall M, Czerwinski Edmund W

机构信息

Sealy Center for Structural Biology, Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-0647, USA.

出版信息

Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1052-4. doi: 10.1107/s0907444903005778. Epub 2003 May 23.

DOI:10.1107/s0907444903005778
PMID:12777771
Abstract

Jun a 1, the major allergen of pollen from the mountain cedar Juniperus ashei, has been crystallized using the hanging-drop vapor-diffusion method at 277 K. The crystals are monoclinic, space group P2(1), with unit-cell parameters a = 53.38, b = 113.48, c = 72.44 A, beta = 96.36 degrees and four molecules in the unit cell. A complete 2.5 A data set has been collected at 100 K with X-rays from a Cu Kalpha rotating-anode generator.

摘要

相似文献

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Crystallization and preliminary X-ray diffraction analysis of Jun a 1, the major allergen isolated from pollen of the mountain cedar Juniperus ashei.
Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1052-4. doi: 10.1107/s0907444903005778. Epub 2003 May 23.
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Crystal structure of Jun a 1, the major cedar pollen allergen from Juniperus ashei, reveals a parallel beta-helical core.来自阿什杜松的主要雪松花粉过敏原Jun a 1的晶体结构揭示了一个平行β-螺旋核心。
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Molecular cloning of the mountain cedar (Juniperus ashei) pollen major allergen, Jun a 1.山地雪松(杜松)花粉主要变应原Jun a 1的分子克隆
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Variable expression of pathogenesis-related protein allergen in mountain cedar (Juniperus ashei) pollen.致病相关蛋白过敏原在山地雪松(杜松)花粉中的表达差异
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Major linear IgE epitopes of mountain cedar pollen allergen Jun a 1 map to the pectate lyase catalytic site.山雪松花粉过敏原Jun a 1的主要线性IgE表位定位于果胶酸裂解酶催化位点。
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Crystal structure of Jun a 1, the major cedar pollen allergen from Juniperus ashei, reveals a parallel beta-helical core.
来自阿什杜松的主要雪松花粉过敏原Jun a 1的晶体结构揭示了一个平行β-螺旋核心。
J Biol Chem. 2005 Feb 4;280(5):3740-6. doi: 10.1074/jbc.M409655200. Epub 2004 Nov 10.