Laing J Hamish E, Wilson George D, Martindale Christine A
RAFT Institute for Plastic Surgery, Mount Vernon Hospital, Northwood, Middlesex, UK.
Melanoma Res. 2003 Jun;13(3):271-7. doi: 10.1097/00008390-200306000-00008.
This prospective study investigated the clinical significance of cell kinetics, measured using bromodeoxyuridine injection and flow cytometry, in primary and metastatic cutaneous malignant melanoma. The findings illustrate that melanoma is a relatively slowly proliferating tumour, with a median potential doubling time (T(pot)) of 8.6 days. There were no significant differences in cell kinetics between primary and secondary disease. Both the duration of the S phase (T(s)) and T(pot), but not the labelling index (LI), showed a correlation with some of the main clinicopathological features in primary disease, including Breslow thickness. On univariate analysis, a short T(pot) and a high LI were associated with shorter disease-free intervals and overall survival for metastatic but not primary lesions. On multivariate analysis, these parameters retained significance when analysed separately with the main clinicopathological variables. These findings suggest that assessment of proliferation in melanoma is able to refine the available prognostic information and identify patients with a poor clinical outcome.
这项前瞻性研究调查了使用溴脱氧尿苷注射和流式细胞术测量的细胞动力学在原发性和转移性皮肤恶性黑色素瘤中的临床意义。研究结果表明，黑色素瘤是一种增殖相对缓慢的肿瘤，中位潜在倍增时间（T(pot)）为8.6天。原发性和继发性疾病的细胞动力学无显著差异。S期持续时间（T(s)）和T(pot)均与原发性疾病的一些主要临床病理特征相关，包括Breslow厚度，但标记指数（LI）与这些特征无关。单因素分析显示，短T(pot)和高LI与转移性而非原发性病变的无病间期缩短和总生存期缩短相关。多因素分析表明，当与主要临床病理变量分别分析时，这些参数仍具有显著性。这些发现表明，评估黑色素瘤的增殖情况能够完善现有的预后信息，并识别出临床结局较差的患者。
