Splichal I, Muneta Y, Mori Y, Takahashi E
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Tokyo, Japan.
J Immunoassay Immunochem. 2003;24(2):219-32. doi: 10.1081/IAS-120020086.
Interleukin 8 (IL-8) is a chemotactic and activating chemokine, especially for neutrophils, which plays an important role in inflammatory process. A pig IL-8 specific enzyme-linked immunosorbent assay (ELISA) was developed to measure IL-8 concentrations in cell culture supernatants and biological fluids. A streptavidin-biotin amplified sandwich method uses mouse capture mAb IZ8.03 and detection biotinylated mouse mAb IZ8.04 against recombinant pig IL-8. The assay specifically and reproducibly recognizes both recombinant and natural pig IL-8. A working range of the assay is 16-1000 pg/mL and takes a mere 3.5 h of incubation time. This pig IL-8 ELISA is a suitable alternative way of measurement of IL-8 concentrations to time consuming and laborious IL-8 bioassays.
白细胞介素8（IL-8）是一种趋化性和活化性趋化因子，对中性粒细胞尤为重要，在炎症过程中发挥着重要作用。开发了一种猪IL-8特异性酶联免疫吸附测定（ELISA）法，用于测量细胞培养上清液和生物体液中的IL-8浓度。一种链霉亲和素-生物素放大夹心方法使用针对重组猪IL-8的小鼠捕获单克隆抗体IZ8.03和检测生物素化小鼠单克隆抗体IZ8.04。该测定法能特异性且可重复地识别重组和天然猪IL-8。该测定法的工作范围为16 - 1000 pg/mL，孵育时间仅需3.5小时。这种猪IL-8 ELISA是一种合适的替代方法，可用于测量IL-8浓度，以替代耗时费力的IL-8生物测定法。
