Náprstková I, Dusek Z, Zemanová Z, Novotná B
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Folia Biol (Praha). 2003;49(2):78-86.
Morphological symptoms of mesonephric kidney damage were analysed in chick embryos treated with nephrotoxic agents--CDDP or DBE. The drugs were administered intraamniotically on ED 3 at doses 0.03 and 0.3 microg CDDP or 100 and 300 microg DBE per embryo. Body weight and absolute and relative measures of the mesonephroi (length, weight and form) were evaluated on ED 10. The higher doses of both agents affected the mass of this organ significantly. Simultaneously, a dose-dependent increase of renal malformations was detected in treated embryos, while the incidence of gross and cardiovascular defects was low (DBE) or absent (CDDP). Together with less pronounced effects on the total body growth, the results gave evidence for a higher sensitivity of the mesonephros to toxic insult when compared to the whole organism. A direct cytotoxic effect multiplied by concomitant injury of blood supply seemed to be the main cause of CDDP nephrotoxicity. In the case of DBE, damage to the mesonephros was probably associated with a primary impairment of the vascular network. The chick embryo in ovo provides a promising system for the assessment of nephrotoxic effects induced by prospective therapeutic agents and environmental contaminants during the prenatal period.
对用肾毒性药物——顺铂（CDDP）或二丁基锡二氯化物（DBE）处理的鸡胚中肾损伤的形态学症状进行了分析。这些药物在胚胎发育第3天经羊膜内给药，剂量为每个胚胎0.03和0.3微克CDDP或100和300微克DBE。在胚胎发育第10天评估体重以及中肾的绝对和相对测量值（长度、重量和形态）。两种药物的较高剂量均显著影响该器官的质量。同时，在处理过的胚胎中检测到肾脏畸形呈剂量依赖性增加，而严重和心血管缺陷的发生率较低（DBE）或未出现（CDDP）。与对全身生长的影响不太明显一起，结果表明与整个生物体相比，中肾对毒性损伤更敏感。直接细胞毒性作用乘以伴随的血液供应损伤似乎是CDDP肾毒性的主要原因。就DBE而言，中肾损伤可能与血管网络的原发性损伤有关。鸡胚在卵内为评估产前期间潜在治疗药物和环境污染物诱导的肾毒性作用提供了一个有前景的系统。
