地塞米松对人卵巢癌细胞系3AO中糖皮质激素受体表达的影响
Effects of Dexamethasone on glucocorticoid receptor expression in a human ovarian carcinoma cell line 3AO.
作者信息
Xu Mingjuan, Song Liangnian, Wang Zhaomei
机构信息
Department of Obstetrics and Gynecology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
出版信息
Chin Med J (Engl). 2003 Mar;116(3):392-5.
OBJECTIVE
To observe the effects of Dexamethasone (Dex), a synthetic glucocorticoid, on glucocorticoid receptor expression in the human ovarian carcinoma cell line 3AO. The molecular mechanism of glucocorticoid (GC) on 3AO cells was also studied.
METHODS
The expression and regulation of the glucocorticoid receptor in 3AO cells was studied by utilizing radioligand binding assay and quantitative RT-PCR.
RESULTS
High affinity and low capacity GR existed in 3AO cells, in addition, GR binding activity was down-regulated by Dex in a time-dependent manner, to a level about 28.34% of control following 24 hours treatment, with a concomitant decrease in GR mRNA. The induction of alkaline phosphatase (AKP) activity by Dex was reversed by RU486, a potent glucocorticoid antagonist.
CONCLUSION
In 3AO cells, functional GR which can be down-regulated by Dex at the protein and mRNA level, exists suggesting that the regulating effects of Dex on GR occur at least partially at the GR mRNA level, and that the cellular effects of Dex on 3AO cells might be mediated by GR.
目的
观察合成糖皮质激素地塞米松（Dex）对人卵巢癌细胞系3AO中糖皮质激素受体表达的影响。同时研究糖皮质激素（GC）作用于3AO细胞的分子机制。
方法
利用放射性配体结合试验和定量逆转录聚合酶链反应（RT-PCR）研究3AO细胞中糖皮质激素受体的表达及调控。
结果
3AO细胞中存在高亲和力、低容量的糖皮质激素受体（GR），此外，GR结合活性被地塞米松以时间依赖性方式下调，处理24小时后降至对照水平的约28.34%，同时GR mRNA减少。地塞米松诱导的碱性磷酸酶（AKP）活性被强效糖皮质激素拮抗剂RU486逆转。
结论
在3AO细胞中，存在可被地塞米松在蛋白质和mRNA水平下调的功能性GR，这表明地塞米松对GR 的调节作用至少部分发生在GR mRNA水平，且地塞米松对3AO细胞的细胞效应可能由GR介导。
Zotero 插件