Carter Charleata A, Jolly David G, Worden Charles E, Hendren Dennis G, Kane Cynthia J M
Research Division, BeluMedX Equine Wound Healing, Little Rock, AR 72212, USA.
Exp Mol Pathol. 2003 Jun;74(3):244-55. doi: 10.1016/s0014-4800(03)00017-0.
Nonhealing wounds of the lower equine limb represent a challenging model. The platelet is a natural source of a myriad of growth factors and cytokines that promote wound healing. This study evaluates the potential of platelet derived factors to enhance wound healing in the lower equine limb. Platelets were isolated from horse blood and activated with thrombin, a process known to induce growth factor release. This produced a platelet gel composed of platelet-rich plasma (PRP). To test this all-natural wound healant, 2.5-cm(2) full thickness cutaneous wounds were created below the knee and hock of a thoroughbred horse. Wounds were treated with PRP gel or left untreated. Sequential wound biopsies collected at Days 7, 36, and 79 postwounding permitted comparison of the temporal expression of differentiation markers and wound repair. To test the hypothesis that wounds treated with PRP gel exhibit more rapid epithelial differentiation and enhanced organization of dermal collagen compared to controls, tissues were stained for cytokeratin 10, a suprabasal differentiation marker, and the reestablishment of collagen was evaluated by trichrome staining. PRP gel-treated wounds at Day 7 expressed intense cytokeratin 10 staining near the wound junction in suprabasal epidermal layers, while staining in control tissues was less intense and restricted to apical epidermal layers distal to the wound junction. By Day 79, the staining was equal in both groups. However, PRP gel-treated wounds at Day 79 contained abundant, dense collagen bundles oriented parallel to each other and to the overlying epithelium, whereas control tissues contained fewer collagen fibers that were oriented randomly. Thus, treatment of wounds with PRP gel induced accelerated epithelial differentiation and produced tissue with organized, interlocking collagen bundles. This study reveals that this novel all-natural wound healant induced wound repair in injuries previously deemed untreatable.
马下肢难愈合伤口是一个具有挑战性的模型。血小板是多种促进伤口愈合的生长因子和细胞因子的天然来源。本研究评估血小板衍生因子促进马下肢伤口愈合的潜力。从马血中分离血小板并用凝血酶激活，这一过程已知可诱导生长因子释放。这产生了一种由富含血小板血浆（PRP）组成的血小板凝胶。为了测试这种纯天然伤口愈合剂，在一匹纯种马的膝盖和跗关节下方制造了2.5平方厘米的全层皮肤伤口。伤口用PRP凝胶治疗或不治疗。在伤口后第7天、36天和79天收集连续的伤口活检样本，以便比较分化标志物的时间表达和伤口修复情况。为了验证与对照组相比，用PRP凝胶治疗的伤口表现出更快的上皮分化和增强的真皮胶原组织化这一假设，对组织进行细胞角蛋白10染色（一种基底上层分化标志物），并通过三色染色评估胶原的重建情况。在第7天，用PRP凝胶治疗的伤口在基底上层表皮层伤口边缘附近表达强烈的细胞角蛋白10染色，而对照组织中的染色较弱，且局限于伤口边缘远端的顶端表皮层。到第79天，两组的染色情况相同。然而，在第79天，用PRP凝胶治疗的伤口含有丰富、密集且相互平行并与上方上皮平行排列的胶原束，而对照组织中的胶原纤维较少且排列随机。因此，用PRP凝胶治疗伤口可诱导加速上皮分化，并产生具有有组织、相互连锁的胶原束的组织。本研究表明，这种新型纯天然伤口愈合剂可诱导此前被认为无法治疗的损伤的伤口修复。
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