通过电子克隆和实验验证鉴定编码趋化因子样因子超家族成员1 - 8（CKLFSF1 - 8）的八个基因。
Identification of eight genes encoding chemokine-like factor superfamily members 1-8 (CKLFSF1-8) by in silico cloning and experimental validation.
作者信息
Han Wenling, Ding Peiguo, Xu Mingxu, Wang Lu, Rui Min, Shi Shuang, Liu Yanan, Zheng Ying, Chen Yingyu, Yang Tian, Ma Dalong
机构信息
Center for Human Disease Genomics, Peking University, 38 Xueyuan Road, Beijing 100083, China.
出版信息
Genomics. 2003 Jun;81(6):609-17. doi: 10.1016/s0888-7543(03)00095-8.
TM4SF11 is only 102 kb from the chemokine gene cluster composed of SCYA22, SCYD1, and SCYA17 on chromosome 16q13. CKLF maps on chromosome 16q22. CKLFs have some characteristics associated with the CCL22/MDC, CX3CL1/fractalkine, CCL17/TARC, and TM4SF proteins. Bioinformatics based on CKLF2 cDNA and protein sequences in combination with experimental validation identified eight novel genes designated chemokine-like factor superfamily members 1-8 (CKLFSF1-8). CKLFSF1-8 form gene clusters; the sequence identities between CKLF2 and CKLFSF1-8 are from 12.5 to 39.7%. Most of the CKLFSFs have alternative RNA splicing forms. CKLFSF1 has a CC motif and higher sequence similarity with chemokines than with any of the other CKLFSFs. CKLFSF8 shares 39.3% amino acid identity with TM4SF11. CKLFSF1 links the CKLFSF family with chemokines, and CKLFSF8 links it with TM4SF. The characteristics of CKLFSF2-7 are intermediate between CKLFSF1 and CKLFSF8. This indicates that CKLFSF represents a novel gene family between the SCY and the TM4SF gene families.
TM4SF11与位于16号染色体16q13上由SCYA22、SCYD1和SCYA17组成的趋化因子基因簇仅相距102 kb。CKLF定位于16号染色体16q22。CKLF具有一些与CCL22/MDC、CX3CL1/趋化素、CCL17/TARC和TM4SF蛋白相关的特征。基于CKLF2 cDNA和蛋白质序列的生物信息学结合实验验证，鉴定出8个新基因，命名为趋化因子样因子超家族成员1 - 8（CKLFSF1 - 8）。CKLFSF1 - 8形成基因簇；CKLF2与CKLFSF1 - 8之间的序列同一性为12.5%至39.7%。大多数CKLFSF具有可变RNA剪接形式。CKLFSF1具有一个CC基序，与趋化因子的序列相似性高于与任何其他CKLFSF的相似性。CKLFSF8与TM4SF11的氨基酸同一性为39.3%。CKLFSF1将CKLFSF家族与趋化因子联系起来，CKLFSF8将其与TM4SF联系起来。CKLFSF2 - 7的特征介于CKLFSF1和CKLFSF8之间。这表明CKLFSF代表了一个介于SCY和TM4SF基因家族之间的新基因家族。
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