Sanderson Andrew, Mitchell Jennie E, Minchin Stephen D, Busby Stephen J W
School of Biosciences, The University of Birmingham, UK.
FEBS Lett. 2003 Jun 5;544(1-3):199-205. doi: 10.1016/s0014-5793(03)00500-3.
Previous work has shown that the base sequence of the DNA segment immediately upstream of the -10 hexamer at bacterial promoters (the extended -10 element) can make a significant contribution to promoter strength. Guided by recently published structural information, we used alanine scanning and suppression mutagenesis of Region 2.4 and Region 3.0 of the Escherichia coli RNA polymerase sigma(70) subunit to identify amino acid sidechains that play a role in recognition of this element. Our study shows that changes in these regions of the sigma(70) subunit can affect the recognition of different extended -10 element sequences.
先前的研究表明，细菌启动子中位于-10六聚体上游紧邻的DNA片段的碱基序列（延伸的-10元件）可对启动子强度产生重大影响。根据最近发表的结构信息，我们对大肠杆菌RNA聚合酶σ⁷⁰亚基的2.4区和3.0区进行丙氨酸扫描和抑制诱变，以鉴定在识别该元件中起作用的氨基酸侧链。我们的研究表明，σ⁷⁰亚基这些区域的变化会影响对不同延伸-10元件序列的识别。
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