Date Isao, Shingo Tetsuro, Ohmoto Takashi
Department of Neurological Surgery, Okayama University Graduate School of Medicine and Dentistry.
Rinsho Shinkeigaku. 2002 Nov;42(11):1144-6.
Due to the development of molecular biology techniques, several types of neurotransmitter or neurotrophic factor secreting cell line can be established. These cell lines were grafted into the brain of animal models of Parkinson's disease and cerebral ischemia after encapsulating into the hollow fiber consisted of semipermeable membrane. Immunological reaction and tumor formation were prevented and functional effects were observed histologically, chemically and behaviorally. Current issues regarding encapsulated cell grafting are: delivery of neurotransmitter and neurotrophic factor simultaneously from one capsule, usage of human-derived cell lines and control of secretion from outside. There are two possible approaches regarding the usage of patient's own neural stem cells for regenerative therapy. Neural stem cells are collected from the subventricular zone of the lateral ventricle and these cells are differentiated into dopaminergic neurons using tyrosine hydroxylase induction cocktail (TH cocktail). Then, these neurons are grafted into the striatum of the patient. Another method is to inject TH cocktail into the patient's striatum in order to induce differentiation of dopaminergic neurons from the neural stem cells in vivo.
由于分子生物学技术的发展，可以建立几种分泌神经递质或神经营养因子的细胞系。将这些细胞系封装到由半透膜组成的中空纤维中后，移植到帕金森病和脑缺血动物模型的大脑中。这样可以防止免疫反应和肿瘤形成，并从组织学、化学和行为学方面观察到功能效果。目前关于封装细胞移植的问题包括：从一个胶囊中同时递送神经递质和神经营养因子、使用人源细胞系以及从外部控制分泌。关于使用患者自身神经干细胞进行再生治疗有两种可能的方法。从侧脑室的室下区收集神经干细胞，然后使用酪氨酸羟化酶诱导混合物（TH混合物）将这些细胞分化为多巴胺能神经元。然后，将这些神经元移植到患者的纹状体中。另一种方法是将TH混合物注射到患者的纹状体中，以在体内诱导神经干细胞分化为多巴胺能神经元。
