Urakami Katsuya, Nakashima Kenji
Section of Environment and Health Science, Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University.
Rinsho Shinkeigaku. 2002 Nov;42(11):1162-4.
The diagnostic significance of tau proteins in cerebrospinal fluids (CSF) has been described in many cases of dementia of Alzheimer type (AD). However, in patients with other diseases showing neurofibrillary tangles (NFT) similar to those in AD, tau proteins in cerebrospinal fluids have not been rigorously investigated. In particular, differentiating corticobasal degeneration (CBD) and progressive supranuclear palsy (PSP) is clinically and pathologically difficult, and a useful biochemical marker to discriminate these two diseases has been a subjects of clinical interest. Therefore, we examined the usefulness of CSF tau and amyloid beta (A beta) proteins for the differentiation between CBD and PSP. CSF total tau (t-tau) and A beta proteins were measured with the sandwich ELISA method (Innogenetics, Belgium). CSF tau protein phosphorylated at serine 199 (p-tau) was measured by a recent established sandwich ELISA (Mitsubishi Chemical Co.). In conclusion, measurement of tau protein levels in CSF may be useful for the differential diagnosis of CBD from PSP. We also suggest that CSF p-tau may be a better biochemical marker than CSF t-tau.
脑脊液（CSF）中tau蛋白的诊断意义已在许多阿尔茨海默病型痴呆（AD）病例中得到描述。然而，在患有其他疾病且出现与AD中相似神经原纤维缠结（NFT）的患者中，脑脊液中的tau蛋白尚未得到严格研究。特别是，区分皮质基底节变性（CBD）和进行性核上性麻痹（PSP）在临床和病理上都很困难，而一种用于区分这两种疾病的有用生化标志物一直是临床关注的课题。因此，我们研究了脑脊液tau蛋白和淀粉样β蛋白（Aβ）在区分CBD和PSP方面的作用。采用夹心ELISA法（比利时Innogenetics公司）检测脑脊液总tau蛋白（t-tau）和Aβ蛋白。采用最近建立的夹心ELISA法（三菱化学公司）检测脑脊液中丝氨酸199位点磷酸化的tau蛋白（p-tau）。总之，检测脑脊液中tau蛋白水平可能有助于CBD与PSP的鉴别诊断。我们还认为，脑脊液p-tau可能是比脑脊液t-tau更好的生化标志物。
