Fukui D, Miyagawa S, Soeda J, Tanaka K, Urayama H, Kawasaki S
First Department of Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.
Eur J Vasc Endovasc Surg. 2003 Jun;25(6):540-5. doi: 10.1053/ejvs.2002.1857.
to examine the expression of transforming growth factor beta1 (TGF-beta1) and the cell kinetics of smooth muscle cells (SMCs) at the neck of abdominal aortic aneurysms (AAAs).
expression of alpha-smooth muscle actin and TGF-beta1 was evaluated by immunostaining, and cell kinetics were estimated by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay and Ki-67 immunostaining in 11 AAAs (at both the dilated region and the neck) and eight occlusive aortas.
the TUNEL-positive SMC ratio in the neck and dilated region was significantly higher than in the occlusive aorta (p<0.01). The percentage of Ki-67-positive SMCs in the neck was significantly higher than in the dilated region (p<0.01) and the occlusive aorta (p=0.032). When compared with the occlusive aorta, the aneurysmal neck had increased TGF-beta1 expression (p=0.01) and reduced SMC density, and the aneurysmal dilated aorta had much more increased TGF-beta1 expression (p<0.01) and much more reduced SMC density (p<0.01).
these results suggest that overexpression of TGF-beta1 might be associated with the reduction of SMC density through SMC apoptosis and reduced proliferative ability of SMCs, leading to dilatation in AAAs.
研究腹主动脉瘤（AAA）颈部转化生长因子β1（TGF-β1）的表达及平滑肌细胞（SMC）的细胞动力学。
通过免疫染色评估α-平滑肌肌动蛋白和TGF-β1的表达，并采用末端脱氧核苷酸转移酶介导的dUTP生物素缺口末端标记法（TUNEL）和Ki-67免疫染色对11例AAA（扩张区域和颈部）和8例闭塞性主动脉进行细胞动力学评估。
颈部和扩张区域TUNEL阳性的SMC比例显著高于闭塞性主动脉（p<0.01）。颈部Ki-67阳性SMC的百分比显著高于扩张区域（p<0.01）和闭塞性主动脉（p=0.032）。与闭塞性主动脉相比，动脉瘤颈部TGF-β1表达增加（p=0.01），SMC密度降低；动脉瘤扩张段主动脉TGF-β1表达增加更为明显（p<0.01），SMC密度降低更为显著（p<0.01）。
这些结果表明，TGF-β1的过度表达可能通过SMC凋亡和SMC增殖能力降低与SMC密度降低有关，从而导致AAA扩张。
