Kubicki J, Ohlenbusch H D, Schroeder E, Wollmer A
Biochemistry. 1976 Dec 28;15(26):5698-702. doi: 10.1021/bi00671a003.
The molecular weight of hemoglobin (III) in monolayers on aqueous subsolutions has been determined by measuring the surface pressure as a function of the protein surface concentration. The dissociation equilibrium between tetrameric and dimeric hemoglobin (III) was determined for spread as well as adsorbed monolayers. The results were compared with analogous measurements in solution. It was found that the numerical value and the pH dependence of the dissociation constant were similar both in the bulk and in the surface phase of the solution. From these findings it was concluded that the native conformation of hemoglobin (III) is retained after adsorption at aqueous surface.
通过测量表面压力作为蛋白质表面浓度的函数，已确定了血红蛋白（III）在水亚溶液上单层中的分子量。对于铺展和吸附的单层，确定了四聚体和二聚体血红蛋白（III）之间的解离平衡。将结果与溶液中的类似测量进行了比较。发现解离常数的数值和pH依赖性在溶液的本体和表面相中都相似。从这些发现得出结论，血红蛋白（III）在水表面吸附后保留了其天然构象。
