Donahoe R M, Huang K Y
Infect Immun. 1976 Apr;13(4):1250-7. doi: 10.1128/iai.13.4.1250-1257.1976.
Previous studies have shown that interferon (IF) preparations enhance phagocytic activity in cultured mouse peritoneal macrophages. It is shown here that cell culture fluids containing large amounts of IF, which had been treated with acid and clarified of the inducer, Newcastle disease virus, enhanced phagocytic activity when injected into mice. Enhanced phagocytic activity also was observed after injection of Newcastle disease virus into mice, but the contribution of IF to this event was unclear. The kinetics of the phagocytic response to inducers in vivo were biphasic. Depression of phagocytosis occurred around 16 to 18 h after injection of Newcastle disease virus. The observed enhancement began about 12 h later and lasted for at least 60 h more. It was concluded that the complexity of the response of mice to an inducer makes analysis of the role of IF in the ensuing events difficult. However, because of documented phagocytosis-enhancing effects of IF in vitro, it is very likely that the in vivo effects observed here are to some degree mediated by IF. On this basis, the concept of the activity of IF as a lymphokine is potentially expanded.
以往的研究表明，干扰素（IF）制剂可增强培养的小鼠腹腔巨噬细胞的吞噬活性。本文表明，含有大量IF的细胞培养液，经酸处理并去除诱导剂新城疫病毒后，注入小鼠体内可增强吞噬活性。给小鼠注射新城疫病毒后也观察到吞噬活性增强，但IF在此过程中的作用尚不清楚。体内对诱导剂的吞噬反应动力学呈双相性。注射新城疫病毒后约16至18小时出现吞噬作用抑制。观察到的增强作用约在12小时后开始，并持续至少60小时以上。得出的结论是，小鼠对诱导剂反应的复杂性使得分析IF在随后事件中的作用变得困难。然而，由于IF在体外具有吞噬增强作用的记录，这里观察到的体内效应很可能在某种程度上是由IF介导的。在此基础上，IF作为淋巴因子的活性概念可能得到扩展。
