Moehring T J, Moehring J M, Stinebring W R
Infect Immun. 1971 Dec;4(6):747-52. doi: 10.1128/iai.4.6.747-752.1971.
It has been reported that treatment with homologous interferon of cells in culture interferes with the process of diphtheria intoxication in these cells. This report both confirms and extends this observation. The most sensitive indicator of diphtheria toxin action in cells, i.e., the inhibition of incorporation of labeled amino acids into protein, was employed, and human, mouse, and chicken interferon-cell systems were studied. Interferon treatment afforded a distinct, reproducible protection from the action of toxin, but in no case could total protection be shown. The "antitoxic" component is stable at pH 2, is species-specific, is not sedimented at 100,000 x g, and is trypsin-sensitive. Human cell preparations are heat-labile. Experiments with cell-free amino acid incorporation systems suggest that the "anti-toxic" effect of interferon is concentrated at the level of the cell membrane and subcellular components are not directly involved.
据报道，在细胞培养中用同源干扰素处理会干扰这些细胞中的白喉中毒过程。本报告证实并扩展了这一观察结果。采用了细胞中白喉毒素作用的最敏感指标，即抑制标记氨基酸掺入蛋白质，并研究了人、小鼠和鸡的干扰素-细胞系统。干扰素处理对毒素的作用提供了明显的、可重复的保护，但在任何情况下都未能显示出完全保护。“抗毒素”成分在pH 2时稳定，具有种属特异性，在100,000×g离心时不沉淀，且对胰蛋白酶敏感。人细胞制剂对热不稳定。无细胞氨基酸掺入系统的实验表明，干扰素的“抗毒素”作用集中在细胞膜水平，亚细胞成分未直接参与。
