Zepeda A, Texier A-C, Gomez J
Departamento de Biotecnología, Div. CBS, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, C.P. 09340, México, DF.
Biotechnol Prog. 2003 May-Jun;19(3):789-93. doi: 10.1021/bp0201408.
The effect of benzene on the nitrifying activity of a sludge produced in steady-state nitrification was evaluated in batch cultures. Benzene at 10 mg/L inhibited nitrate formation by 53%, whereas at 5 mg/L there was no inhibition. For initial benzene concentrations of 0, 7, and 10 mg/L, the specific rates of NO(3)(-)-N production were 0.545 +/- 0.101, 0.306 +/- 0.024, and 0.141 +/- 0.010 g NO(3)(-)-N/g microbial protein-N.h, respectively. The specific rates of benzene consumption at 7, 12, and 20 mg/L were 0.034 +/- 0.003, 0.050 +/- 0.006, and 0.027 +/- 0.002 g/g microbial protein-N.h, respectively. Up to a concentration of 10 mg/L, benzene was first oxidized to phenol, which was later totally oxidized to acetate. Benzene at higher concentrations (20 and 30 mg/L) was converted to intermediates other than acetate, phenol, or catechol. These results suggest that this type of nitrifying consortium coupled with a denitrification system may have promising applications for complete removal of nitrogen and benzene from wastewaters.
在分批培养中评估了苯对稳态硝化过程中产生的污泥硝化活性的影响。10mg/L的苯使硝酸盐形成受到53%的抑制，而5mg/L时则没有抑制作用。对于初始苯浓度为0、7和10mg/L的情况，NO₃⁻-N的特定生成速率分别为0.545±0.101、0.306±0.024和0.141±0.010g NO₃⁻-N/g微生物蛋白-N·h。7、12和20mg/L时苯的特定消耗速率分别为0.034±0.003、0.050±0.006和0.027±0.002g/g微生物蛋白-N·h。在浓度达到10mg/L之前，苯首先被氧化为苯酚，随后苯酚被完全氧化为乙酸盐。较高浓度（20和30mg/L）的苯被转化为除乙酸盐、苯酚或邻苯二酚之外的中间产物。这些结果表明，这种类型的硝化菌群与反硝化系统相结合可能在从废水中完全去除氮和苯方面具有广阔的应用前景。
