Starkey K J, Janezic A, Jones G, Jordan N, Baker G, Ludgate M
Department of Medicine, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, Wales, UK.
J Mol Endocrinol. 2003 Jun;30(3):369-80. doi: 10.1677/jme.0.0300369.
The thyrotrophin receptor (TSHR) provides an autoantigenic link between the thyroid and orbit in Graves' (GD) and thyroid eye diseases (TED). We measured TSHR transcripts in different fat depots to determine whether TSHR expression levels are influenced by the autoimmune/inflammatory process and/or thyroid hormone status, using quantitative real-time PCR. Nine intact or fractionated adipose samples, from patients with GD and/or TED, were analysed ex vivo. Eight expressed the TSHR, at levels approaching the thyroid, and one was at the limit of detection. Thirteen/fifteen orbital and abdominal fat samples from patients free of GD and TED, measured ex vivo, were negative for TSHR transcripts and two were at the limit of detection. All preadipocyte samples induced to differentiate in vitro expressed the TSHR. To investigate the influence of thyroid hormone status on adipose TSHR expression, we induced hyper- and hypothyroidism in BALBc mice by administering tri-iodothyronine and propylthiouracil respectively. In euthyroid animals, whole fat samples were at the limit of detection and were not altered by thyroid hormone status. The results show that adipose TSHR expression ex vivo indicates adipogenesis in progress in vivo and is associated with the autoimmune/inflammatory process in GD and TED but is not restricted to the orbit or influenced by thyroid hormone status.
促甲状腺激素受体（TSHR）在格雷夫斯病（GD）和甲状腺眼病（TED）中为甲状腺与眼眶之间提供了一个自身抗原联系。我们使用定量实时聚合酶链反应来测量不同脂肪库中的TSHR转录本，以确定TSHR表达水平是否受自身免疫/炎症过程和/或甲状腺激素状态的影响。对9份来自GD和/或TED患者的完整或分离的脂肪样本进行了离体分析。其中8份样本的TSHR表达水平接近甲状腺，1份样本的表达水平处于检测下限。对13/15份来自无GD和TED患者的眼眶和腹部脂肪样本进行离体测量，其中TSHR转录本呈阴性，2份样本的表达水平处于检测下限。所有体外诱导分化的前脂肪细胞样本均表达TSHR。为了研究甲状腺激素状态对脂肪组织TSHR表达的影响，我们分别给BALBc小鼠注射三碘甲状腺原氨酸和丙硫氧嘧啶，诱导其发生甲状腺功能亢进和减退。在甲状腺功能正常的动物中，全脂肪样本的表达水平处于检测下限，且不受甲状腺激素状态的影响。结果表明，离体脂肪组织TSHR表达表明体内存在正在进行的脂肪生成，并且与GD和TED中的自身免疫/炎症过程相关，但不仅限于眼眶，也不受甲状腺激素状态的影响。
