Bezkorovainy A, Grohlich D, Sly D A
Int J Pept Protein Res. 1976;8(3):291-3. doi: 10.1111/j.1399-3011.1976.tb02506.x.
Human serum transferrin was fragmented by N-bromosuccinimide and reduction-alkylation. It was observed that there were at least two each of tryptophanyl-serine and tryptophanyl-aspartic acid, and one each of tryptophanyl-alanine and tryptophanyl-glutamic acid bonds. The size of fragments detected by polyacrylamide gel electrophoresis ranged from 8,000 to 70,000 daltons. Several of the fragments were isolated in a homogeneous form with respect to molecular weight, but were shown to be mixtures of at least five molecular species each by end group analysis.
人血清转铁蛋白经N-溴代琥珀酰亚胺裂解及还原烷基化处理。观察到色氨酸-丝氨酸和色氨酸-天冬氨酸各至少有两个，色氨酸-丙氨酸和色氨酸-谷氨酸各有一个键。通过聚丙烯酰胺凝胶电泳检测到的片段大小范围为8000至70000道尔顿。其中几个片段以分子量均一的形式分离出来，但通过端基分析表明每个片段至少是五种分子种类的混合物。
