Taylor N S, Hasubski A T, Fox J G, Lee A
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge 02139.
J Med Microbiol. 1992 Nov;37(5):299-303. doi: 10.1099/00222615-37-5-299.
Thirty-five Helicobacter pylori isolates, 21 H. mustelae isolates and four strains of H. felis were compared for their ability to agglutinate red blood cells (RBCs). Isolates were examined in a slide haemagglutination assay with RBCs from 11 animal species, including rodents, carnivores and primates, as well as man. RBCs were agglutinated by 65-90% of H. mustelae isolates and 16-57% of H. pylori isolates. Treatment of H. mustelae with pronase and heat inhibited haemagglutination (HA) whereas heating only of H. pylori inhibited HA. Treatment of all strains of H. mustelae with trypsin inhibited agglutination of human RBCs; 75% of the treated strains did not agglutinate ferret RBCs. These results suggested that protein(s) may be important haemagglutinins for these bacteria. Variable HA profiles together with varying results after treatment of RBCs with fetuin, D-mannose, and neuraminidase suggested that multiple receptors may be involved in HA reactions with H. pylori and H. mustelae. The observation that H. mustelae and H. pylori agglutinated RBCs of several species and closely adhered to gastric epithelium supported the hypothesis that adherence plays a role in the colonisation and pathogenicity of H. mustelae and H. pylori. H. felis did not adhere to gastric epithelium and did not agglutinate RBCs of any species; nevertheless, H. felis can readily colonise and produce gastritis in several mammals.
对35株幽门螺杆菌分离株、21株鼬獾幽门螺杆菌分离株和4株猫幽门螺杆菌菌株进行了红细胞（RBC）凝集能力的比较。在玻片血凝试验中，使用来自11种动物（包括啮齿动物、食肉动物、灵长类动物以及人类）的红细胞对这些分离株进行检测。65% - 90%的鼬獾幽门螺杆菌分离株和16% - 57%的幽门螺杆菌分离株能凝集红细胞。用链霉蛋白酶和加热处理鼬獾幽门螺杆菌会抑制血凝作用（HA），而仅加热幽门螺杆菌则会抑制HA。用胰蛋白酶处理所有鼬獾幽门螺杆菌菌株会抑制人红细胞的凝集；75%的处理菌株不能凝集雪貂红细胞。这些结果表明蛋白质可能是这些细菌重要的血凝素。不同的HA谱以及用胎球蛋白、D - 甘露糖和神经氨酸酶处理红细胞后得到的不同结果表明，多种受体可能参与了幽门螺杆菌和鼬獾幽门螺杆菌的HA反应。鼬獾幽门螺杆菌和幽门螺杆菌能凝集几种动物的红细胞并紧密黏附于胃上皮细胞，这一观察结果支持了黏附在鼬獾幽门螺杆菌和幽门螺杆菌的定植及致病性中起作用这一假说。猫幽门螺杆菌不黏附于胃上皮细胞，也不能凝集任何物种的红细胞；然而，猫幽门螺杆菌能轻易地在几种哺乳动物中定植并引发胃炎。
