Hancock Kathy, Khan Azra, Williams Fatima B, Yushak Melinda L, Pattabhi Sowmya, Noh John, Tsang Victor C W
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA.
J Clin Microbiol. 2003 Jun;41(6):2577-86. doi: 10.1128/JCM.41.6.2577-2586.2003.
The Western blot for cysticercosis, which uses lentil lectin purified glycoprotein (LLGP) antigens extracted from the metacestode of Taenia solium, has been the "gold standard" serodiagnostic assay since it was first described in 1989. We report that the diagnostic antigens at 14, 18, and 21 kDa, as well as some larger disulfide-bonded antigens, are actually all members of a very closely related family of proteins, the 8-kDa antigens. The genes for 18 unique, mature proteins have been identified. Nine of these were chemically synthesized and tested in an enzyme-linked immunosorbent assay with a battery of defined serum samples, including 32 cysticercosis-positive serum samples reactive with the 8-kDa antigens of LLGP on Western blotting, 34 serum samples from patients with other parasitic infections, and 15 normal human serum samples. One of the 8-kDa antigens, TsRS1, is 100% sensitive and 100% specific. TsRS1 will be one component of a cocktail of three to four synthetic or recombinant antigens, based on the diagnostic bands of the Western blot, which will be used for the serodiagnosis of cysticercosis.
自1989年首次描述以来,用于囊尾蚴病的蛋白质印迹法一直是“金标准”血清诊断检测方法,该方法使用从猪带绦虫的中绦期提取的扁豆凝集素纯化糖蛋白(LLGP)抗原。我们报告称,14 kDa、18 kDa和21 kDa的诊断抗原,以及一些较大的二硫键连接抗原,实际上都是一个密切相关的蛋白质家族——8 kDa抗原的成员。已鉴定出18种独特成熟蛋白的基因。其中9种经化学合成,并在酶联免疫吸附测定中与一系列明确的血清样本进行检测,这些样本包括32份在蛋白质印迹法中与LLGP的8 kDa抗原反应的囊尾蚴病阳性血清样本、34份来自其他寄生虫感染患者的血清样本以及15份正常人血清样本。8 kDa抗原之一TsRS1的敏感性和特异性均为100%。基于蛋白质印迹法的诊断条带,TsRS1将成为由三到四种合成或重组抗原组成的混合物中的一种成分,该混合物将用于囊尾蚴病的血清诊断。