应用重组抗原和合成抗原的快速酶联免疫吸附试验对人体猪囊尾蚴病的血清学诊断。
Serologic diagnosis of human Taenia solium cysticercosis by using recombinant and synthetic antigens in QuickELISA™.
机构信息
Division of Parasitic Diseases and Malaria, and Scientific Resource Program, Coordinating Center for Infectious Diseases, Center for Global Health, Centers for Diseases Control and Prevention, 1600 Clifton Road, Atlanta, GA 30329, USA.
出版信息
Am J Trop Med Hyg. 2011 Apr;84(4):587-93. doi: 10.4269/ajtmh.2011.10-0079.
Diagnosis of Taenia solium cysticercosis is an important component in the control and elimination of cysticercosis and taeniasis. New detection assays using recombinant and synthetic antigens originating from the lentil lectin-purified glycoproteins (LLGPs) of T. solium cysticerci were developed in a QuickELISA™ format. We analyzed a panel of 474 serum samples composed of 108 serum samples from donors with two or more viable cysts, 252 serum samples from persons with other parasitic infections, and 114 serum samples from persons with no documented illnesses. The sensitivities and specificities of T24H QuickELISA™, GP50 QuickELISA™, and Ts18var1 QuickELISA™ were 96.3% and 99.2%, 93.5% and 98.6%, and 89.8% and 96.4%, respectively, for detecting cases with multiple, viable cysts. T24H QuickELISA™ performs best among the three assays, and has sensitivity and specificity values comparable to those of the LLGP enzyme-linked immunosorbent blot. The QuickELISA™ are simple, rapid quantitative methods for detecting antibodies specific for T. solium cysticerci antigens.
猪带绦虫囊尾蚴病的诊断是控制和消灭囊尾蚴病和带绦虫病的重要组成部分。使用源自猪带绦虫囊尾蚴的扁豆凝集素纯化糖蛋白（LLGPs）的重组和合成抗原开发了新的快速酶联免疫吸附试验（QuickELISA）检测方法。我们分析了一个由 474 个血清样本组成的面板，其中包括 108 个来自有两个或更多存活囊肿的供体的血清样本、252 个来自患有其他寄生虫感染的人的血清样本和 114 个来自没有记录的疾病的人的血清样本。T24H QuickELISA、GP50 QuickELISA 和 Ts18var1 QuickELISA 检测多个、存活囊肿病例的敏感性和特异性分别为 96.3%和 99.2%、93.5%和 98.6%以及 89.8%和 96.4%。T24H QuickELISA 在这三种检测方法中表现最好，其敏感性和特异性值与 LLGP 酶联免疫吸附试验相当。QuickELISA 是检测针对猪带绦虫囊尾蚴抗原的特异性抗体的简单、快速定量方法。
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