Kwara Awewura, Schiro Ronald, Cowan Lauren S, Hyslop Newton E, Wiser Mark F, Roahen Harrison Stephanie, Kissinger Patricia, Diem Lois, Crawford Jack T
Department of Tropical Medicine, Tulane University School of Public Health and Tropical Medicine, New Orleans, Louisiana 70112, USA.
J Clin Microbiol. 2003 Jun;41(6):2683-5. doi: 10.1128/JCM.41.6.2683-2685.2003.
Spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem repeat analysis (MIRU-VNTR) were evaluated for the ability to differentiate 64 Mycobacterium tuberculosis isolates from 10 IS6110-defined clusters. MIRU-VNTR performed slightly better than spoligotyping in reducing the number of clustered isolates and the sizes of the clusters. All epidemiologically related isolates remained clustered by MIRU-VNTR but not by spoligotyping.
对间隔寡核苷酸分型(Spoligotyping)和分枝杆菌散布重复单位可变数目串联重复序列分析(MIRU-VNTR)区分来自10个由IS6110定义的簇的64株结核分枝杆菌分离株的能力进行了评估。在减少聚集分离株的数量和簇的大小时,MIRU-VNTR的表现略优于间隔寡核苷酸分型。所有流行病学相关的分离株通过MIRU-VNTR仍保持聚集状态,但通过间隔寡核苷酸分型则不然。