United Kingdom Health Protection Agency, London UK.
Emerg Infect Dis. 2010 Feb;16(2):272-80. doi: 10.3201/eid1602.091032.
To inform development of tuberculosis (TB) control strategies, we characterized a total of 2,261 Mycobacterium tuberculosis complex isolates by using multiple phenotypic and molecular markers, including polymorphisms in repetitive sequences (spoligotyping and variable-number tandem repeats [VNTRs]) and large sequence and single-nucleotide polymorphisms. The Beijing family was strongly associated with multidrug resistance (p = 0.0001), and VNTR allelic variants showed strong associations with spoligotyping families: >or=5 copies at exact tandem repeat (ETR) A, >or=2 at mycobacterial interspersed repetitive unit 24, and >or=3 at ETR-B associated with the East African-Indian and M. bovis strains. All M. tuberculosis isolates were differentiated into 4 major lineages, and a maximum parsimony tree was constructed suggesting a more complex phylogeny for M. africanum. These findings can be used as a model of pathogen global diversity.
为了制定结核病（TB）控制策略，我们使用多种表型和分子标记物（包括重复序列（ spoligotyping 和可变数串联重复 [VNTR]）和大序列和单核苷酸多态性）对总共 2261 株结核分枝杆菌复合体分离株进行了特征分析。北京家族与耐多药密切相关（p=0.0001），VNTR 等位基因变体与 spoligotyping 家族密切相关：在精确串联重复（ETR）A 处>或=5 个拷贝，在分枝杆菌插入重复单元 24 处>或=2 个拷贝，在 ETR-B 处>或=3 个拷贝与东非-印度和 M. bovis 株有关。所有结核分枝杆菌分离株分为 4 个主要谱系，构建的最大简约树表明 M. africanum 的系统发育更为复杂。这些发现可用作病原体全球多样性的模型。
