Dalle Frédéric, Roz Pascale, Dautin Guillaume, Di-Palma Marc, Kohli Evelyne, Sire-Bidault C, Fleischmann Marie George, Gallay Anne, Carbonel Sylvia, Bon Fabienne, Tillier Claude, Beaudeau Pascal, Bonnin Alain
Laboratoire de Parasitologie Mycologie et Laboratoire de Microbiologie Médicale et Moléculaire (EA 562), CHU et Faculté de Médecine, Dijon, France.
J Clin Microbiol. 2003 Jun;41(6):2690-3. doi: 10.1128/JCM.41.6.2690-2693.2003.
In September 2001, a waterborne outbreak of gastroenteritis occurred in eastern France. Of 31 fecal samples from symptomatic individuals, 19 tested positive for Cryptosporidium with two PCRs targeting the Hsp70 and the 18S rRNA genes of CRYPTOSPORIDIUM: Sequencing of the PCR fragments produced sequences identical to that of Cryptosporidium parvum genotype 1.
2001年9月,法国东部发生了一起水源性肠胃炎疫情。在对有症状个体采集的31份粪便样本中,通过针对隐孢子虫热休克蛋白70(Hsp70)基因和18S核糖体RNA(rRNA)基因的两种聚合酶链式反应(PCR)检测,有19份样本隐孢子虫呈阳性:对PCR片段进行测序后得到的序列与微小隐孢子虫基因型1的序列相同。