Girardin Stephen E, Boneca Ivo G, Carneiro Leticia A M, Antignac Aude, Jéhanno Muguette, Viala Jérôme, Tedin Karsten, Taha Muhamed-Kheir, Labigne Agnes, Zähringer Ulrich, Coyle Anthony J, DiStefano Peter S, Bertin John, Sansonetti Philippe J, Philpott Dana J
Unité de Pathogénie Microbienne Moléculaire, INSERM U389, Institut Pasteur, 28, Rue du Dr. Roux, 75724Paris Cedex 15, France.
Science. 2003 Jun 6;300(5625):1584-7. doi: 10.1126/science.1084677.
Although the role of Toll-like receptors in extracellular bacterial sensing has been investigated intensively, intracellular detection of bacteria through Nod molecules remains largely uncharacterized. Here, we show that human Nod1 specifically detects a unique diaminopimelate-containing N-acetylglucosamine-N-acetylmuramic acid (GlcNAc-MurNAc) tripeptide motif found in Gram-negative bacterial peptidoglycan, resulting in activation of the transcription factor NF-kappaB pathway. Moreover, we show that in epithelial cells (which represent the first line of defense against invasive pathogens), Nod1is indispensable for intracellular Gram-negative bacterial sensing.
尽管Toll样受体在细胞外细菌感知中的作用已得到深入研究，但通过Nod分子对细菌进行细胞内检测在很大程度上仍未得到充分表征。在此，我们表明人类Nod1可特异性检测革兰氏阴性菌肽聚糖中独特的含二氨基庚二酸的N-乙酰葡糖胺-N-乙酰胞壁酸（GlcNAc-MurNAc）三肽基序，从而激活转录因子NF-κB途径。此外，我们表明在上皮细胞（代表抵御侵袭性病原体的第一道防线）中，Nod1对于细胞内革兰氏阴性菌的感知不可或缺。
