Geng Hong, Meng Ziqiang
Institute of Environmental Medicine and Toxicology, Shanxi University, Taiyuan 030006, China.
Wei Sheng Yan Jiu. 2003 Mar;32(2):103-6.
By spectrophotometric method, the level of non-enzymatic antioxidant glutathione (GSH) and the ratio of GSH/GSSG were examined in nine organs of mice of both sexes after exposed to sulphur dioxide (SO2) at several concentrations. The results showed that: (1) at 22 mg/m3 of SO2 inhalation concentration, there were no significant difference between the experimental and control mice for the GSH levels and GSH/GSSG ratio in all of tested organs; (2) at 64 mg/m3, the GSH levels were significantly decreased in lungs, kidney, brain, stomach and testicle of the male mice in the experimental group, while the GSH levels and GSH/GSSG ratios in the tested organs of the female mice had no marked changes in the comparison with those in the control group; (3) 148 mg/m3 of SO2 inhalation led to decrease of both the GSH levels and GSH/GSSG ratios, in that the GSH levels were significantly lowered in liver, lung, kidney, heart, brain, stomach, small intestine and testicle of the male mice and in liver, lung, kidney, small intestine of the female mice, and the GSH/GSSG ratios were in liver, kidney, small intestine and testicle of the male mice and in liver, lung, kidney, brain, stomach, small intestine of the female mice. It indicated that SO2 above certain concentration could make effects not only on respiratory organ, but also on other organs, and the susceptibility of the male and the female mice to SO2 was different. The decline of the GSH level and GSH/GSSH ratio suggests that the toxic effect of SO2 is associated with its lowering the levels of antioxidants and weakening the defense system and antioxidation in organisms.
采用分光光度法，检测了不同浓度二氧化硫（SO₂）暴露后雌雄小鼠九个器官中非酶抗氧化剂谷胱甘肽（GSH）的水平及GSH/GSSG比值。结果表明：（1）吸入浓度为22mg/m³的SO₂时，实验组与对照组小鼠各检测器官的GSH水平及GSH/GSSG比值均无显著差异；（2）吸入浓度为64mg/m³时，实验组雄性小鼠的肺、肾、脑、胃和睾丸中GSH水平显著降低，而雌性小鼠各检测器官的GSH水平及GSH/GSSG比值与对照组相比无明显变化；（3）吸入浓度为148mg/m³的SO₂导致GSH水平及GSH/GSSG比值均下降，其中雄性小鼠的肝、肺、肾、心、脑、胃、小肠和睾丸以及雌性小鼠的肝、肺、肾、小肠中GSH水平显著降低，雄性小鼠的肝、肾、小肠和睾丸以及雌性小鼠的肝、肺、肾、脑、胃、小肠中GSH/GSSG比值下降。这表明一定浓度以上的SO₂不仅会对呼吸器官产生影响，还会对其他器官产生影响，且雌雄小鼠对SO₂的易感性不同。GSH水平及GSH/GSSH比值的下降表明SO₂的毒性作用与其降低抗氧化剂水平、削弱机体防御系统和抗氧化能力有关。
