一种改良的辣根过氧化物酶（HRP）方法，用于追踪成年动物外周神经再生早期阶段整装标本中的轴突。
An improved HRP method for tracing axons in whole-mount preparations during early stages of regeneration in peripheral nerves of adult animals.
作者信息
Tonge D, Edbladh M, Edström A, Kanje M
机构信息
Division of Biomedical Sciences, King's College, London, UK.
出版信息
J Neurosci Methods. 1992 Aug;44(1):27-31. doi: 10.1016/0165-0270(92)90110-y.
PMID:1279320
Abstract
An improved method using HRP for visualizing axons in whole-mount preparations is described. Application of this method to preparations of regenerating frog peripheral nerves maintained in organ culture was used to show that regeneration following nerve crush begins after about 3 days.
摘要
描述了一种使用辣根过氧化物酶（HRP）在整装标本中可视化轴突的改进方法。将该方法应用于在器官培养中维持的再生青蛙外周神经标本，以表明神经挤压后的再生在大约3天后开始。
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