Sutherland Betsy M, Bennett Paula V, Cintron-Torres Nela, Hada Megumi, Trunk John, Monteleone Denise, Sutherland John C, Laval Jacques, Stanislaus Marisha, Gewirtz Alan
Biology Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.
J Radiat Res. 2002 Dec;43 Suppl:S149-52. doi: 10.1269/jrr.43.s149.
Ionizing radiation induces clusters of DNA damages--oxidized bases, abasic sites and strand breaks--on opposing strands within a few helical turns. Such damages have been postulated to be difficult to repair, as are double strand breaks (one type of cluster). We have shown that low doses of low and high linear energy transfer (LET) radiation induce such damage clusters in human cells. In human cells, DSB are about 30% of the total of complex damages, and the levels of DSBs and oxidized pyrimidine clusters are similar. The dose responses for cluster induction in cells can be described by a linear relationship, implying that even low doses of ionizing radiation can produce clustered damages. Studies are in progress to determine whether clusters can be produced by mechanisms other than ionizing radiation, as well as the levels of various cluster types formed by low and high LET radiation.
电离辐射会在几个螺旋圈的相对链上诱导产生DNA损伤簇——氧化碱基、无碱基位点和链断裂。据推测，此类损伤难以修复，双链断裂（一种损伤簇类型）也是如此。我们已经表明，低剂量的低线性能量传递（LET）和高线性能量传递辐射会在人类细胞中诱导产生此类损伤簇。在人类细胞中，双链断裂约占复杂损伤总数的30%，双链断裂和氧化嘧啶簇的水平相似。细胞中损伤簇诱导的剂量反应可用线性关系来描述，这意味着即使是低剂量的电离辐射也能产生成簇损伤。目前正在进行研究，以确定损伤簇是否能通过电离辐射以外的机制产生，以及低LET和高LET辐射形成的各种损伤簇类型的水平。
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