Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Molecular Medicine Research Center, Institute of Biomedicine, Tabriz University of Medical Sciences, Tabriz, Iran.
J Appl Clin Med Phys. 2023 Feb;24(2):e13879. doi: 10.1002/acm2.13879. Epub 2022 Dec 22.
Nanoscopic lesions (complex damages), are the most lethal lesions for the cells. As nanoparticles have become increasingly popular in radiation therapy and the importance of analyzing nanoscopic dose enhancement has increased, a reliable tool for nanodosimetry has become indispensable. In this regard, the DNA plasmid is a widely used tool as a nanodosimetry probe in radiobiology and nano-radiosensitization studies. This approach is helpful for unraveling the radiosensitization role of nanoparticles in terms of physical and physicochemical effects and for quantifying radiation-induced biological damage. This review discusses the potential of using plasmid DNA assays for assessing the relative effects of nano-radiosensitizers, which can provide a theoretical basis for the development of nanoscopic biodosimetry and nanoparticle-based radiotherapy.
纳米级损伤（复杂损伤）是细胞最致命的损伤。随着纳米颗粒在放射治疗中的应用越来越广泛，分析纳米级剂量增强的重要性也越来越大，因此可靠的纳米剂量学工具变得不可或缺。在这方面，DNA 质粒作为一种纳米剂量学探针，在放射生物学和纳米增敏研究中被广泛应用。这种方法有助于揭示纳米颗粒在物理和物理化学效应方面的增敏作用，并定量评估辐射引起的生物损伤。本文综述了使用质粒 DNA 测定法评估纳米增敏剂相对效应的潜力，这可为纳米尺度生物剂量学和基于纳米颗粒的放射治疗的发展提供理论基础。
