Nelson Gregory A, Jones Tamako A, Chesnut Aaron, Smith Anna L
Loma Linda University Radiobiology Program, 11175 Campus St., Chan Shun Pavilion A1010 Loma Linda, California 92354, USA.
J Radiat Res. 2002 Dec;43 Suppl:S199-203. doi: 10.1269/jrr.43.s199.
We used the nematode C. elegans to characterize the genotoxic and cytotoxic effects of ionizing radiation in a simple animal model emphasizing the unique effects of charged particle radiation. Here we demonstrate by RT-PCR differential display and whole genome microarray hybridization experiments that gamma rays, accelerated protons and iron ions at the same physical dose lead to unique transcription profiles. 599 of 17871 genes analyzed (3.4%) showed differential expression 3 hrs after exposure to 3 Gy of radiation. 193 were up-regulated, 406 were down-regulated and 90% were affected only by a single species of radiation. A novel statistical clustering technique identified the regulatory relationships between the radiation-modulated genes and showed that genes affected by each radiation species were associated with unique regulatory clusters. This suggests that independent homeostatic mechanisms are activated in response to radiation exposure as a function of track structure or ionization density.
我们利用线虫秀丽隐杆线虫在一个简单的动物模型中表征电离辐射的遗传毒性和细胞毒性作用，重点研究带电粒子辐射的独特效应。在此，我们通过逆转录聚合酶链反应（RT-PCR）差异显示和全基因组微阵列杂交实验证明，在相同物理剂量下，γ射线、加速质子和铁离子会导致独特的转录谱。在分析的17871个基因中，有599个（3.4%）在暴露于3戈瑞辐射3小时后显示出差异表达。其中193个基因上调，406个基因下调，90%的基因仅受单一辐射种类的影响。一种新的统计聚类技术确定了辐射调节基因之间的调控关系，并表明受每种辐射种类影响的基因与独特的调控簇相关联。这表明，作为径迹结构或电离密度的函数，独立的稳态机制在辐射暴露时被激活。
