Straczkowski Marek, Kowalska Irina, Nikolajuk Agnieszka, Dzienis-Straczkowska Stella, Szelachowska Malgorzata, Kinalska Ida
Department of Endocrinology, Diabetology and Internal Medicine, Medical University, Bialystok, Poland.
Cardiovasc Diabetol. 2003 May 16;2:5. doi: 10.1186/1475-2840-2-5.
Interleukin 8 (IL-8) is a cytokine with atherogenic properties. In vitro studies revealed that it is produced and secreted by human adipocytes. We recently reported that plasma IL-8 is increased in obese subjects with normal glucose tolerance (NGT). The aim of the present study was to measure plasma IL-8 concentrations in subjects with impaired glucose tolerance (IGT).
A total of 44 subjects with marked overweight or obesity (BMI > 27.8 kg/m2), 27 with NGT and 17 with IGT, were recruited for the present study. Plasma IL-8 levels were measured in fasting state, after an oral glucose tolerance test (OGTT) and after euglycemic hyperinsulinemic clamp.
The studied groups did not differ in fasting IL-8 concentrations. Both OGTT and clamp resulted in a significant increase in plasma IL-8. The change in IL-8 after clamp was similar in both groups. In contrast, after OGTT plasma IL-8 levels (IL-8OGTT) were markedly higher in IGT individuals. In IGT, but not NGT group, IL-8OGTT was positively related to postload glucose and negatively to insulin sensitivity.
Plasma IL-8 concentrations after glucose load are increased in obese IGT subjects in comparison to normoglycemic weight-matched individuals. Increase in plasma IL-8 might be both insulin-mediated (during clamp) and glucose-mediated (during OGTT).
白细胞介素8（IL-8）是一种具有致动脉粥样硬化特性的细胞因子。体外研究表明，它由人脂肪细胞产生并分泌。我们最近报道，糖耐量正常（NGT）的肥胖受试者血浆IL-8升高。本研究的目的是测量糖耐量受损（IGT）受试者的血浆IL-8浓度。
本研究共招募了44名明显超重或肥胖（BMI>27.8kg/m2）的受试者，其中27名糖耐量正常，17名糖耐量受损。在空腹状态、口服葡萄糖耐量试验（OGTT）后和正常血糖高胰岛素钳夹试验后测量血浆IL-8水平。
研究组空腹IL-8浓度无差异。OGTT和钳夹试验均导致血浆IL-8显著升高。两组钳夹试验后IL-8的变化相似。相比之下，OGTT后IGT个体的血浆IL-8水平（IL-8OGTT）明显更高。在IGT组而非NGT组中，IL-8OGTT与负荷后血糖呈正相关，与胰岛素敏感性呈负相关。
与血糖正常、体重匹配的个体相比，肥胖IGT受试者葡萄糖负荷后血浆IL-8浓度升高。血浆IL-8的升高可能是胰岛素介导的（在钳夹试验期间）和葡萄糖介导的（在OGTT期间）。
