Kontny Ewa, Rudnicka Weronika, Kowalczewski Jacek, Marcinkiewicz Janusz, Maslinski Wlodzimierz
Department of Pathophysiology and Immunology, Institute of Rheumatology, Warsaw, Poland.
Arthritis Rheum. 2003 Jun;48(6):1551-5. doi: 10.1002/art.10975.
To investigate the effects of taurine chloramine (Tau-Cl), a chlorinated derivative of the amino acid taurine, on the expression of cyclooxygenase (COX) isoenzymes and prostaglandin E(2) (PGE(2)) synthesis in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS).
FLS, isolated from the synovial tissue of RA patients, were treated in vitro with either interleukin-1beta (IL-1beta; 1 ng/ml) alone or together with 200-500 microM Tau-Cl. The expression of COX isoenzymes was evaluated at both the protein (Western blotting) and the messenger RNA (mRNA) (reverse transcriptase-polymerase chain reaction) levels. The concentration of PGE(2) was measured by competitive acetylcholinesterase enzyme immunoassay.
Resting FLS expressed mRNA encoding both COX-1 and COX-2, but only COX-1 was present at the protein level. These cells produced negligible amounts of PGE(2). Upon stimulation with IL-1beta, elevation of COX-2, but not COX-1, mRNA and protein preceded the enhancement of PGE(2) synthesis. In the presence of 300-400 microM Tau-Cl, significant inhibition of IL-1beta-triggered COX-2 mRNA and protein, and a related decrease in PGE(2) production, was observed. In contrast, no significant changes in COX-1 mRNA and protein levels were noted.
Tau-Cl inhibits IL-1beta-triggered elevation of COX-2 and generation of PGE(2) by RA FLS. These results expand the spectrum of known antiinflammatory activities of this compound.
研究牛磺酸氯胺（Tau-Cl，氨基酸牛磺酸的一种氯化衍生物）对类风湿关节炎（RA）成纤维样滑膜细胞（FLS）中环氧合酶（COX）同工酶表达及前列腺素E2（PGE2）合成的影响。
从RA患者滑膜组织中分离出FLS，在体外分别用单独的白细胞介素-1β（IL-1β；1 ng/ml）或与200 - 500 μM Tau-Cl共同处理。通过蛋白质（蛋白质印迹法）和信使核糖核酸（mRNA）（逆转录-聚合酶链反应）水平评估COX同工酶的表达。采用竞争性乙酰胆碱酯酶酶免疫测定法测量PGE2的浓度。
静息的FLS表达编码COX-1和COX-2的mRNA，但在蛋白质水平仅存在COX-1。这些细胞产生的PGE2量可忽略不计。在用IL-1β刺激后，COX-2而非COX-1的mRNA和蛋白质升高先于PGE2合成的增强。在存在300 - 400 μM Tau-Cl的情况下，观察到对IL-1β触发的COX-2 mRNA和蛋白质有显著抑制，以及PGE2产生相关减少。相比之下，COX-1 mRNA和蛋白质水平未观察到显著变化。
Tau-Cl抑制RA FLS中IL-1β触发的COX-2升高和PGE2生成。这些结果扩展了该化合物已知抗炎活性的范围。
