酿酒酵母第三条染色体上一个新基因的验证

Verification of a new gene on Saccharomyces cerevisiae chromosome III.

作者信息

Zhang Zhihong, Dietrich Fred S

机构信息

Department of Molecular Genetics and Microbiology, Center for Genome Technology, Duke University Medical Center, Durham, NC 27710, USA.

出版信息

Yeast. 2003 Jun;20(8):731-8. doi: 10.1002/yea.996.

DOI:10.1002/yea.996

PMID:12794934

Abstract

We present verification of the existence of a previously unannotated, intron-containing gene of 134 amino acids (predicted molecular weight approximately 15.5 kDa) located on chromosome III of Saccharomyces cerevisiae. Strains carrying a deletion of this gene, which we have called YCL012C, reveal no apparent phenotype. Orthologues of YCL012C are present in related species S. bayanus, S. paradoxus and Ashbya gossypii. Comparison with other fungal sequences reveals that orthologues of this gene are likely present in Schizosaccharomyces pombe, Neurospora crassa and Cryptococcus neoformans as well, indicating that YCL012C is a widely conserved fungal gene.

摘要

我们展示了酿酒酵母III号染色体上一个先前未注释的、含内含子的134个氨基酸（预测分子量约15.5 kDa）基因存在的验证。携带该基因缺失的菌株（我们将其命名为YCL012C）未表现出明显的表型。YCL012C的直系同源基因存在于相关物种巴氏酵母、奇异酵母和棉阿舒囊霉中。与其他真菌序列的比较表明，该基因的直系同源基因可能也存在于粟酒裂殖酵母、粗糙脉孢菌和新型隐球菌中，这表明YCL012C是一个广泛保守的真菌基因。