酵母三号染色体的完整DNA序列。

The complete DNA sequence of yeast chromosome III.

作者信息

Oliver S G, van der Aart Q J, Agostoni-Carbone M L, Aigle M, Alberghina L, Alexandraki D, Antoine G, Anwar R, Ballesta J P, Benit P

机构信息

Manchester Biotechnology Centre, UMIST, UK.

出版信息

Nature. 1992 May 7;357(6373):38-46. doi: 10.1038/357038a0.

DOI:10.1038/357038a0

PMID:1574125

Abstract

The entire DNA sequence of chromosome III of the yeast Saccharomyces cerevisiae has been determined. This is the first complete sequence analysis of an entire chromosome from any organism. The 315-kilobase sequence reveals 182 open reading frames for proteins longer than 100 amino acids, of which 37 correspond to known genes and 29 more show some similarity to sequences in databases. Of 55 new open reading frames analysed by gene disruption, three are essential genes; of 42 non-essential genes that were tested, 14 show some discernible effect on phenotype and the remaining 28 have no overt function.

摘要

啤酒酵母三号染色体的完整DNA序列已被测定。这是对任何生物体的整条染色体进行的首次完整序列分析。这条315千碱基的序列揭示了182个编码长度超过100个氨基酸的蛋白质的开放阅读框，其中37个与已知基因相对应，另外29个与数据库中的序列有一定相似性。在通过基因破坏分析的55个新开放阅读框中，有3个是必需基因；在测试的42个非必需基因中，14个对表型有一定可察觉的影响，其余28个没有明显功能。