Lefebvre O, Carles C, Conesa C, Swanson R N, Bouet F, Riva M, Sentenac A
Département de Biologie Cellulaire et Moléculaire, Centre d'Etudes de Saclay, Gif sur Yvette, France.
Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10512-6. doi: 10.1073/pnas.89.21.10512.
Yeast transcription factor IIIC (TFIIIC) is a multisubunit protein complex that interacts with two control elements of class III promoters called the A and B blocks. Here we describe the gene encoding the 138-kDa subunit (tau 138), which is involved in B-block binding. From the DNA sequence, the open reading frame, interrupted by an intron with an unusual 3' splice acceptor site, is in agreement with all the microsequencing data for peptides within tau 138. TFC3 is a single-copy gene located on chromosome I; it is essential for cell viability as shown by a gene disruption experiment. Epitope-tagging of the TFC3 gene product and DNA binding experiments are consistent with the presence of one copy of tau 138 in TFIIIC-DNA complexes.
酵母转录因子IIIC（TFIIIC）是一种多亚基蛋白复合物，它与III类启动子的两个控制元件（称为A和B框）相互作用。在此，我们描述了编码138 kDa亚基（tau 138）的基因，该亚基参与B框结合。根据DNA序列，被一个具有不寻常3'剪接受体位点的内含子打断的开放阅读框，与tau 138内肽段的所有微量测序数据一致。TFC3是位于染色体I上的单拷贝基因；基因破坏实验表明它对细胞活力至关重要。TFC3基因产物的表位标记和DNA结合实验与TFIIIC-DNA复合物中存在一个tau 138拷贝一致。
