Varla-Leftherioti Marighoula, Spyropoulou-Vlachou Maria, Niokou Demetra, Keramitsoglou Theodora, Darlamitsou Areti, Tsekoura Christina, Papadimitropoulos Miltiadis, Lepage Virginia, Balafoutas Christos, Stavropoulos-Giokas Catherine
Immunology Department and RSA Clinic, Helena Venizelou Maternity Hospital, Athens, Greece.
Am J Reprod Immunol. 2003 Mar;49(3):183-91. doi: 10.1034/j.1600-0897.2003.00018.x.
Natural killer (NK) cell receptors (NKRs) have been suggested to protect trophoblast, but their function at the fetomaternal interface remains unknown. To investigate if the outcome of pregnancy depends on women's NKRs, we studied the NKR repertoire in couples with recurrent spontaneous abortions (RSA).
Twenty-six childless couples with > or = 2 abortions, characterized by alloimmune abnormalities, and 26 control couples were genotyped for five killer immunoglobulin-like receptors (KIR) and two CD94/NKG receptors, known to have as ligands human leukocyte antigen (HLA) class I molecules with trophoblastic expression: inhibitory 2DL1,2,3 and activating 2DS1,4 KIRs, inhibitory NKG2A and activating NKG2C. Detected repertoires of women and partners were compared between the two groups.
Less aborters than controls were found to have all three inhibitory KIRs (30.77% versus 69.23%, P = 0.01), some of them had only one inhibitory KIR (19.23% versus 3.85%, P = 0.08) and most of them were lacking inhibitory KIRs possessed by their husbands (57.69% versus 15.38%, P = 0.001).
Women with alloimmune abortions have a limited inhibiting KIR repertoire and such miscarriages may occur because trophoblastic HLA class I molecules are recognized by decidual NK cells lacking the appropriate inhibitory KIRs.
自然杀伤（NK）细胞受体（NKRs）被认为可保护滋养层细胞，但其在母胎界面的功能仍不清楚。为研究妊娠结局是否取决于女性的NKRs，我们对反复自然流产（RSA）夫妇的NKR库进行了研究。
26对有≥2次流产且伴有同种免疫异常的无子女夫妇以及26对对照夫妇，针对5种杀伤细胞免疫球蛋白样受体（KIR）和2种CD94/NKG受体进行基因分型，已知这些受体以滋养层表达的人类白细胞抗原（HLA）I类分子作为配体：抑制性的2DL1、2、3和激活性的2DS1、4 KIRs，抑制性的NKG2A和激活性的NKG2C。比较两组中女性及其配偶检测到的受体库。
与对照组相比，流产者中拥有全部三种抑制性KIRs的较少（30.77%对69.23%，P = 0.01），其中一些仅有一种抑制性KIR（19.23%对3.85%，P = 0.08），并且她们中的大多数缺乏丈夫所拥有的抑制性KIRs（57.69%对15.38%，P = 0.001）。
患有同种免疫性流产的女性具有有限的抑制性KIR库，此类流产可能是因为缺乏适当抑制性KIRs的蜕膜NK细胞识别了滋养层HLA I类分子。
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