在生育和复发性流产女性诱导的蜕膜自然杀伤细胞培养物中，KIR、ILT 抑制性受体和 VEGF 产生的表达差异。
Differences in the Expression of KIR, ILT Inhibitory Receptors, and VEGF Production in the Induced Decidual NK Cell Cultures of Fertile and RPL Women.
机构信息
Department of Clinical Immunology, Transplantation Institute, Medical University of Warsaw, Nowogrodzka 59, Warsaw, 02-006 Mazovian Voivodeship, Poland.
Department of Gynecology and Gynecologic Oncology, Military Institute of Health Sciences, Szaserów 128, Warsaw, 04-141 Mazovian Voivodeship, Poland.
出版信息
Biomed Res Int. 2021 May 4;2021:6673427. doi: 10.1155/2021/6673427. eCollection 2021.
RESULTS
KIR2DL1 and ILT-2 expression on idNK cells was higher in healthy women than in RPL patients. Sildenafil enhanced NKG2A expression in RPL patients. VEGF concentration was higher in fertile woman idNK cell cultures. idNK cells were more sensitive for necrosis in RPL than in fertile women. SC did not influence VEGF production or idNK cell apoptosis.
CONCLUSIONS
A combination of hypoxia, IL-15, and AZA promotes the conversion of pbNK into idNK cells CD56CD16-expressing KIR receptors and produces VEGF. Alterations in KIR2DL1 and ILT-2 expression as well as impaired VEGF production were associated with RPL. SC affects NKG2A expression on RPL idNK cells. SC had no effect on VEGF release or idNK cell apoptosis.
结果
健康女性中 idNK 细胞上的 KIR2DL1 和 ILT-2 表达高于 RPL 患者。西地那非增强了 RPL 患者的 NKG2A 表达。在生育能力正常的女性 idNK 细胞培养物中，VEGF 浓度更高。与生育能力正常的女性相比，RPL 患者的 idNK 细胞对坏死更敏感。SC 不影响 VEGF 产生或 idNK 细胞凋亡。
结论
缺氧、IL-15 和 AZA 的组合促进了 pbNK 向表达 KIR 受体的 idNK 细胞（CD56CD16）的转化，并产生了 VEGF。KIR2DL1 和 ILT-2 表达的改变以及 VEGF 产生受损与 RPL 有关。SC 影响 RPL idNK 细胞上的 NKG2A 表达。SC 对 VEGF 释放或 idNK 细胞凋亡没有影响。
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