Jamieson C V, MacDonald A G
Department of Biomedical Sciences, University of Aberdeen, Aberdeen AB24 2TZ, Scotland, UK.
Undersea Hyperb Med. 2002 Winter;29(4):286-93.
A method of patch clamp recording in hyperbaric oxygen is described which is derived from a method for patch clamping at high hydrostatic pressure. Excised patches equilibrate with hyperbaric oxygen within 10 minutes. Experiments with BK channels expressed in HEK 293 cells showed that 1 MPa O2 caused an increase in the ionic permeability of the patch, an effect accelerated in the presence of ferrous ions. Seal resistance was unaffected. Small negative currents, (VH - 40 to - 65 mV, symmetrical KCl solutions), apparent in skewed all-points amplitude histograms, reduced the signal to noise ratio. In some patches the BK channel activity was inhibited and in others it persisted with the currents becoming irresolvable in the increased noise.
本文描述了一种在高压氧环境下进行膜片钳记录的方法，该方法源自一种在高静水压力下进行膜片钳操作的方法。切除的膜片在10分钟内与高压氧达到平衡。对在HEK 293细胞中表达的BK通道进行的实验表明，1 MPa的氧气会导致膜片的离子通透性增加，在亚铁离子存在的情况下，这种效应会加速。封接电阻不受影响。在倾斜的全点幅度直方图中明显出现的小负电流（VH为 - 40至 - 65 mV，对称KCl溶液）降低了信噪比。在一些膜片中，BK通道活性受到抑制，而在另一些膜片中，其活性持续存在，电流在增加的噪声中变得无法分辨。
