Inoue H, Cho J Y, Satodate H, Sakashita M, Hidaka E, Fukami S, Kazawa T, Yoshida T, Shiokawa A, Kudo S
Digestive Disease Center, Dept. of Pathology, Showa University Northern Yokohama Hospital, Yokohama, Japan.
Scand J Gastroenterol Suppl. 2003(237):37-9. doi: 10.1080/00855910310001485.
The aim of this project is to acquire a direct image of histology from in vivo gastrointestinal mucosa. In other words, the task of 'endo-microscope' is to observe the cellular architecture of tissue in vivo during routine endoscopic examination. As the first step to completing this study, resected fresh specimens from the oesophagus. stomach and colon were examined by laser-scanning confocal microscopy (LCM) (Fluoview, Olympus, Tokyo). Fresh untreated mucosal specimens obtained by endoscopic pinch biopsy, polypectomy or endoscopic mucosal resection were collected and placed in normal saline and examined by LCM, collecting the reflective light of a 488-nm wavelength argon laser beam. As the second step, a probe-type LCM 'endo-microscope' was designed and applied to observe the human oral-cavity mucosa. The probe has 4.5-mm outer diameter and 20-cm length, which enables easy access to oral cavity mucosa. The estimated special resolution of the probe is 1-5 microm. A real-time microscopic image directly from ex vivo fresh specimens was acquired. The acquired LCM images corresponded well with the conventional H-E light microscopic images. Cell wall, nucleus and cytoplasm were simultaneously visualized by LCM scanning. This novel method enables serial imaginary microscopic sections on fresh specimens. In addition, a probe-type LCM 'endo-microscope' was designed and was applied to observe human oral cavity mucosa. Virtual histological images from the living oral squamous cell were successfully obtained. LCM images from ex vivo fresh specimens demonstrated the features of the H-E staining histological image. In the next step to accomplish our project, we developed a LCM probe with 4.5-mm outer diameter to obtain a virtual image of human oral cavity mucosa.
本项目的目的是获取体内胃肠道黏膜的组织学直接图像。换句话说，“内镜显微镜”的任务是在常规内镜检查过程中对体内组织的细胞结构进行观察。作为完成本研究的第一步，对取自食管、胃和结肠的新鲜切除标本进行激光扫描共聚焦显微镜（LCM）检查（Fluoview，奥林巴斯，东京）。通过内镜钳取活检、息肉切除术或内镜黏膜切除术获得的未经处理的新鲜黏膜标本被收集起来，置于生理盐水中，然后用LCM进行检查，收集488纳米波长氩激光束的反射光。作为第二步，设计并应用了一种探头式LCM“内镜显微镜”来观察人体口腔黏膜。该探头外径为4.5毫米，长度为20厘米，便于进入口腔黏膜。该探头的估计空间分辨率为1 - 5微米。直接从离体新鲜标本获取了实时微观图像。所获取的LCM图像与传统的苏木精 - 伊红（H - E）光学显微镜图像非常吻合。通过LCM扫描可同时观察到细胞壁、细胞核和细胞质。这种新方法能够对新鲜标本进行连续的虚拟微观切片。此外，设计并应用了一种探头式LCM“内镜显微镜”来观察人体口腔黏膜。成功获得了来自活体口腔鳞状细胞的虚拟组织学图像。离体新鲜标本的LCM图像显示出H - E染色组织学图像的特征。为完成我们的项目，下一步我们开发了一种外径为4.5毫米的LCM探头，以获取人体口腔黏膜的虚拟图像。
