用于分离分枝杆菌的BACTEC MGIT 960系统与固体培养基的比较评估

Comparative evaluation of the BACTEC MGIT 960 system with solid medium for isolation of mycobacteria.

作者信息

Lee J J, Suo J, Lin C B, Wang J D, Lin T Y, Tsai Y C

机构信息

Department of Internal Medicine, Buddhist Tzu Chi General Hospital, Hualien, Hualien County, Taiwan.

出版信息

Int J Tuberc Lung Dis. 2003 Jun;7(6):569-74.

PMID:12797700

Abstract

SETTING

The utilisation of new, rapid methods of diagnosis of tuberculosis is currently of great interest for tuberculosis control. This study was carried out in a teaching hospital in the eastern region of Taiwan.

OBJECTIVE

The BACTEC MGIT 960 system was evaluated and compared with Lowenstein-Jensen (LJ) medium and Middlebrook 7H11 plate for recovery rate and time to detection of mycobacteria.

DESIGN

A total of 1396 sputum samples were tested for the presence of mycobacteria. Specimens were processed and inoculated separately in the BACTEC MGIT 960 system, on LJ medium and 7H11 for comparative study.

RESULTS

The BACTEC MGIT 960 detected 235 isolates (100%), followed by LJ with 205 isolates (87.2%) and 7H11 with 178 isolates (75.7%). The mean time to detection of Mycobacterium tuberculosis complex was 11.6 days with MGIT 960, 20.1 days with LJ, and 18.7 days with 7H11. The contamination rates were 15.1% with MGIT 960, 10.1% with LJ and 9.7% with 7H11.

CONCLUSION

The BACTEC MGIT 960 system is a sensitive, rapid mycobacterial culturing system. However, the high contamination rate is a concern that should be carefully evaluated in the clinical setting.

摘要

背景

新型快速结核病诊断方法的应用目前对于结核病控制具有重大意义。本研究在台湾东部地区的一家教学医院开展。

目的

对BACTEC MGIT 960系统进行评估，并与罗氏（LJ）培养基和Middlebrook 7H11平板在分枝杆菌回收率和检测时间方面进行比较。

设计

共对1396份痰标本进行分枝杆菌检测。标本分别在BACTEC MGIT 960系统、LJ培养基和7H11平板上进行处理和接种，以进行对比研究。

结果

BACTEC MGIT 960检测出235株分离株（100%），其次是LJ培养基检测出205株（87.2%），7H11平板检测出178株（75.7%）。结核分枝杆菌复合群的平均检测时间，MGIT 960为11.6天，LJ为20.1天，7H11为18.7天。污染率方面，MGIT 960为15.1%，LJ为10.1%，7H11为9.7%。