Rivera A B, Tupasi T E, Grimaldo E R, Cardano R C, Co V M
Tropical Disease Foundation, Makati Medical Center, Makati City, Philippines.
Int J Tuberc Lung Dis. 1997 Oct;1(5):454-9.
Clinical microbiology laboratory with limited resources in a developing country.
To determine the recovery rate of Mycobacterium tuberculosis (MTB) in Mycobacteria Growth Indicator Tube (MGIT) combined with Löwenstein Jensen (LJ) culture medium.
Stock cultures and reference strains of mycobacteria and clinical specimens were inoculated into MGIT and onto LJ. The combined recovery rate was determined and time to positive culture in each medium was compared.
All known stock and reference cultures of mycobacteria grew in both media. MGIT combined with LJ increased the recovery rate from 109 (63.4%) to 122 (70.9%) of 172 clinical specimens. Of those isolated, the yield in MGIT (99.2%) exceeded that in LJ (89.3%). The average day to detection of MTB in MGIT was earlier by 14.2 days compared to LJ (15.7 days vs. 29.9 days). For mycobacteria other than tuberculosis (MOTT), there was little difference in the recovery time, except for M. kansasii where growth in MGIT was earlier by one week and M. triviale where growth in MGIT was detected later than LJ.
MGIT is an excellent system for the rapid isolation of mycobacteria. It increases the recovery rate of MTB when combined with LJ.
在一个发展中国家资源有限的临床微生物实验室。
确定分枝杆菌生长指示管（MGIT）联合罗-琴（LJ）培养基中结核分枝杆菌（MTB）的回收率。
将分枝杆菌的储备培养物、参考菌株及临床标本接种到MGIT和LJ培养基上。确定联合回收率，并比较每种培养基中培养阳性所需时间。
所有已知的分枝杆菌储备培养物和参考培养物在两种培养基中均生长。MGIT联合LJ使172份临床标本的回收率从109份（63.4%）提高到122份（70.9%）。在分离出的菌株中，MGIT的培养阳性率（99.2%）超过LJ（89.3%）。MGIT检测到MTB的平均天数比LJ早14.2天（15.7天对29.9天）。对于非结核分枝杆菌（MOTT），除堪萨斯分枝杆菌在MGIT中生长早一周，平凡分枝杆菌在MGIT中生长比LJ晚外，回收率差异不大。
MGIT是快速分离分枝杆菌的优良系统。与LJ联合使用时可提高MTB的回收率。
