通过电化学蘸笔纳米光刻技术将组氨酸标签蛋白固定在镍上。

Immobilization of histidine-tagged proteins on nickel by electrochemical dip pen nanolithography.

作者信息

Agarwal Gunjan, Naik Rajesh R, Stone Morley O

机构信息

Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Ohio 45433, USA.

出版信息

J Am Chem Soc. 2003 Jun 18;125(24):7408-12. doi: 10.1021/ja029856p.

DOI:10.1021/ja029856p

PMID:12797815

Abstract

Dip-pen nanolithography (DPN) is becoming a popular technique to "write" molecules on a surface by using the tip of an atomic force microscope (AFM) coated with the desired molecular "ink". In this work, we demonstrate that poly-histidine-tagged peptides and proteins, and free-base porphyrins coated on AFM probes, can be chelated to ionized regions on a metallic nickel surface by applying an electric potential to the AFM tip in the DPN process. DPN has been accomplished in the Tapping Mode of AFM, which creates many possible applications of positioning and subsequently imaging biomolecules, especially on soft surfaces.

摘要

蘸笔纳米光刻技术（DPN）正成为一种通过使用涂有所需分子“墨水”的原子力显微镜（AFM）尖端在表面“书写”分子的流行技术。在这项工作中，我们证明了在DPN过程中，通过向AFM尖端施加电势，涂覆在AFM探针上的聚组氨酸标记的肽和蛋白质以及游离碱卟啉可以螯合到金属镍表面的电离区域。DPN是在AFM的轻敲模式下完成的，这为生物分子的定位及后续成像创造了许多可能的应用，尤其是在柔软表面上。