Department of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Nanoscale Res Lett. 2007 Aug 4;2(8):373-84. doi: 10.1007/s11671-007-9083-3.
Bio-nanopatterning of surfaces is a very active interdisciplinary field of research at the interface between biotechnology and nanotechnology. Precise patterning of biomolecules on surfaces with nanometre resolution has great potential in many medical and biological applications ranging from molecular diagnostics to advanced platforms for fundamental studies of molecular and cell biology. Bio-nanopatterning technology has advanced at a rapid pace in the last few years with a variety of patterning methodologies being developed for immobilising biomolecules such as DNA, peptides, proteins and viruses at the nanoscale on a broad range of substrates. In this review, the status of research and development are described, with particular focus on the recent advances on the use of nanolithographic techniques as tools for biomolecule immobilisation at the nanoscale. Present strengths and weaknesses, as well future challenges on the different nanolithographic bio-nanopatterning approaches are discussed.
表面的生物纳米图案化是生物技术和纳米技术之间的一个非常活跃的交叉学科领域。在从分子诊断到分子和细胞生物学基础研究的高级平台等众多医学和生物学应用中，具有纳米分辨率的生物分子在表面上的精确图案化具有巨大的潜力。在过去的几年中，生物纳米图案化技术发展迅速，开发了多种图案化方法，用于将生物分子（如 DNA、肽、蛋白质和病毒）在纳米尺度上固定在各种基底上。在这篇综述中，描述了研究和发展的现状，特别关注了纳米光刻技术作为纳米尺度生物分子固定化工具的最新进展。讨论了不同纳米光刻生物纳米图案化方法的当前优势和劣势以及未来挑战。
