Zhang Wen, Xie Yunfeng, Ai Shiyun, Wan Fangli, Wang Jian, Jin Litong, Jin Jiye
Department of Chemistry, East China Normal University, ZhingShan Road North 3663, Shanghai 200062, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jul 5;791(1-2):217-25. doi: 10.1016/s1570-0232(03)00227-7.
The fabrication and application of a novel electrochemical detection (ED) method with the functionalized multi-wall carbon nanotubes (MWNTs) chemically modified electrode (CME) for liquid chromatography (LC) were described. The electrochemical behaviors of dopamine (DA) and other monoamine neurotransmitters at the CME were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results indicated that the CME exhibited efficient electrocatalytic effects on the current responses of monoamine neurotransmitters and their metabolites with high sensitivity, high stability and long-life activity. In LC-ED, DA, norepinephrine (NE), 3-methoxy-4-hydroxyphenylglycol (MHPG), 3,4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA) had good and stable current responses at the CME. The linear ranges of seven analytes were over four orders of magnitude and the detection limits were 2.5 x 10(-10) mol/l for DA, 2.5 x 10(-10) mol/l for NE, 5.0 x 10(-10) mol/l for MHPG, 3.0 x 10(-10) mol/l for DOPAC, 3.5 x 10(-10) mol/l for 5-HT, 6.0 x 10(-10) mol/l for 5-HIAA, 1.25 x 10(-9) mol/l for HVA. The application of this method coupled with microdialysis sampling for the determination of monoamine neurotransmitters and their metabolites in Parkinsonian patients' cerebrospinal fluid was satisfactory.
描述了一种用于液相色谱（LC）的新型电化学检测（ED）方法的制备及应用，该方法采用功能化多壁碳纳米管（MWNTs）化学修饰电极（CME）。通过循环伏安法（CV）和差分脉冲伏安法（DPV）研究了多巴胺（DA）和其他单胺类神经递质在CME上的电化学行为。结果表明，CME对单胺类神经递质及其代谢物的电流响应表现出高效的电催化作用，具有高灵敏度、高稳定性和长寿命活性。在LC-ED中，DA、去甲肾上腺素（NE）、3-甲氧基-4-羟基苯乙二醇（MHPG）、3,4-二羟基苯乙酸（DOPAC）、5-羟色胺（5-HT）、5-羟吲哚乙酸（5-HIAA）和高香草酸（HVA）在CME上具有良好且稳定的电流响应。七种分析物的线性范围超过四个数量级，检测限分别为：DA为2.5×10⁻¹⁰ mol/l，NE为2.5×10⁻¹⁰ mol/l，MHPG为5.0×10⁻¹⁰ mol/l，DOPAC为3.0×10⁻¹⁰ mol/l，5-HT为3.5×10⁻¹⁰ mol/l，5-HIAA为6.0×10⁻¹⁰ mol/l，HVA为1.25×10⁻⁹ mol/l。该方法与微透析采样相结合用于测定帕金森病患者脑脊液中单胺类神经递质及其代谢物的应用效果令人满意。
