Fundueanu Gheorghe, Constantin Marieta, Mihai Doina, Bortolotti Fabrizio, Cortesi Rita, Ascenzi Paolo, Menegatti Enea
Department of Pharmaceutical Sciences, University of Ferrara, Via Fossato di Mortara, 17-19, I-44100 Ferrara, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jul 5;791(1-2):407-19. doi: 10.1016/s1570-0232(03)00258-7.
Pullulan microspheres containing cyclodextrin (CyD) were obtained by chemical crosslinking with epichlorohydrin of an alkaline solution of pullulan (Pul) and alpha-, beta- or gamma-CyD. The amount of alpha-, beta- and gamma-CyD in microspheres was 120, 156, and 138 micromol/g, respectively, as determined from the percentage of iodine incorporated in the hydrophobic cavity of CyD's. Microspheres were packed in a glass column and the liquid chromatographic behaviour by isocratic elution of different drugs or typical organic compounds (TOC), taken as model drugs, was investigated. The increase of the retention volume (V(R)) of each compound, depending on the interaction(s) between CyD's cavity and the considered molecule, is characterized by a broadening of the peaks. The interaction coefficient K, corresponding to the ratio between the V(R) value of each tested molecule on Pul-alpha-, Pul-beta- and Pul-gamma-CyD active stationary phase and the V(R) value of benzoic acid on St/maltodextrin neutral stationary phase, was determined. According to K values, the accurate prediction can be done on the potential drugs to be conditioned in suitable CyD cavity. Values of K allow to anticipate the release profiles of drugs considered.
