体外人类精子功能所需的钙

Calcium requirements for human sperm function in vitro.

作者信息

Marín-Briggiler Clara I, Gonzalez-Echeverría Fernanda, Buffone Mariano, Calamera Juan C, Tezón Jorge G, Vazquez-Levin Mónica H

机构信息

Instituto de Biología y Medicina Experimental, CONICET-UBA, Buenos Aires, Argentina.

出版信息

Fertil Steril. 2003 Jun;79(6):1396-403. doi: 10.1016/s0015-0282(03)00267-x.

DOI:10.1016/s0015-0282(03)00267-x

PMID:12798888

Abstract

OBJECTIVE

To determine extracellular calcium (Ca(2+)) requirements for the maintenance of human sperm function in vitro.

DESIGN

Prospective study.

SETTING

Basic research laboratory.

PATIENT(S): Normozoospermic volunteers provided fresh semen samples; follicular fluid (human FF) and oocytes were collected from women undergoing IVF-ET.

INTERVENTION(S): Spermatozoa were incubated for </=18 hours in media containing different CaCl(2) concentrations (maximum, 2.5 mM [control]).

MAIN OUTCOME MEASURES

Protein tyrosine phosphorylation patterns, development of hyperactivated motility, induction of the acrosome reaction (AR) in response to human FF, and sperm interaction with homologous zona pellucida (ZP).

RESULT(S): Cells maintained for 18 hours in medium containing >/=0.1 mM of Ca(2+) were able to undergo the AR when exposed to human FF in the presence of 2.5 mM of Ca(2+). Calcium concentrations of >/=0.22 mM were sufficient to reach protein tyrosine phosphorylation levels and hyperactivated motility values similar to those of controls. Higher Ca(2+) concentrations (>/=0.58 mM) were required to produce maximum human FF-induced AR in previously capacitated cells and to obtain an adequate sperm-ZP binding.

CONCLUSION(S): Different steps of the fertilization process have distinctive Ca(2+) requirements. Whereas 0.22 mM of Ca(2+) is sufficient for the development of some capacitation-related events, human FF-induced AR and sperm-ZP interaction require 0.58 mM of this cation.

摘要

目的

确定体外维持人类精子功能所需的细胞外钙（Ca(2+)）水平。

设计

前瞻性研究。

地点

基础研究实验室。

患者

提供新鲜精液样本的正常精子志愿者；从接受体外受精-胚胎移植（IVF-ET）的女性中收集卵泡液（人卵泡液，human FF）和卵母细胞。

干预措施

将精子在含有不同氯化钙（CaCl(2)）浓度（最高2.5 mM [对照组]）的培养基中孵育≤18小时。

主要观察指标

蛋白质酪氨酸磷酸化模式、超活化运动的发展、对人卵泡液（human FF）反应诱导的顶体反应（AR）以及精子与同源透明带（ZP）的相互作用。

结果

在含有≥0.1 mM Ca(2+)的培养基中维持18小时的细胞，在2.5 mM Ca(2+)存在下暴露于人卵泡液（human FF）时能够发生顶体反应（AR）。钙浓度≥0.22 mM足以达到与对照组相似的蛋白质酪氨酸磷酸化水平和超活化运动值。在先前获能的细胞中，需要更高的Ca(2+)浓度（≥0.58 mM）才能产生最大的人卵泡液（human FF）诱导的顶体反应（AR）并获得足够的精子-透明带（ZP）结合。