Lee Ji-Yun, Kim Tae-Yun, Gan Xiao-Xian, Kang Shin-Yong, Hong Sung-Jong
Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul 156-756, South Korea.
Parasitol Int. 2003 Jun;52(2):175-8. doi: 10.1016/s1383-5769(03)00002-3.
A recombinant pore-forming peptide of Clonorchis sinensis, clonorin, was evaluated for serodiagnostic reagent of clonorchiasis by enzyme-linked immunosorbent assay detecting IgG antibody. Recombinant clonorin showed 100% specificity and low sensitivity for sera of human clonorchiasis. In contrast, C. sinensis crude antigen revealed lower specificity and higher sensitivity than recombinant clonorin did. In sera of experimental rabbits, clonorin-specific IgG antibody was increased remarkably 8 weeks after the infection and retained around level of OD(490)=0.2 for 1 year. With excellent antigenic specificity, it is suggested that the recombinant clonorin can be used as an ingredient of the cocktail antigen for serodiagnosis of clonorchiasis from early stages of the infection.
通过酶联免疫吸附试验检测IgG抗体，对重组华支睾吸虫成孔肽clonorin进行了华支睾吸虫病血清诊断试剂评估。重组clonorin对人类华支睾吸虫病血清显示出100%的特异性和低敏感性。相比之下，华支睾吸虫粗抗原的特异性低于重组clonorin，而敏感性更高。在实验兔血清中，感染后8周clonorin特异性IgG抗体显著增加，并在1年内保持在OD(490)=0.2左右的水平。鉴于其优异的抗原特异性，建议重组clonorin可作为混合抗原的成分用于华支睾吸虫病感染早期的血清诊断。
