Matsumoto M, Kanouchi H, Suzuki K, Kaneki K, Kawasaki Y, Oka T
Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan.
Comp Biochem Physiol B Biochem Mol Biol. 2003 Jun;135(2):255-62. doi: 10.1016/s1096-4959(03)00073-3.
We isolated a perchloric acid soluble protein from the post-mitochondria supernatant fraction of the rat lung and designated it as RLu-PSP1. The protein is soluble in 5% perchloric acid and was purified by ammonium sulfate fractionation and CM-Sephadex chromatography. The amino acid sequence of RLu-PSP was identical with that of rat liver PSP (RL-PSP). RLu-PSP inhibited protein synthesis in a rabbit reticulocyte lysate system. It was expressed mainly in cytoplasm of bronchioles and alveolar epithelial cells of the lung from 60-day-old rats. In 15-day-old rat embryos, the epithelial-lining of the terminal buds of the respiratory tree was immunopositive. The expression of RLu-PSP increased from the embryonic 15th day to the postnatal 40th day. This is the first report on the presence of a PSP in rat lung and on its involvement in the regulation of cellular growth and differentiation.
我们从大鼠肺线粒体后上清液组分中分离出一种高氯酸可溶性蛋白，并将其命名为RLu - PSP1。该蛋白可溶于5%的高氯酸，通过硫酸铵分级分离和CM - 葡聚糖凝胶色谱法进行纯化。RLu - PSP的氨基酸序列与大鼠肝脏PSP（RL - PSP）相同。RLu - PSP在兔网织红细胞裂解物系统中抑制蛋白质合成。它主要在60日龄大鼠肺的细支气管和肺泡上皮细胞的细胞质中表达。在15日龄大鼠胚胎中，呼吸树终末芽的上皮衬里呈免疫阳性。RLu - PSP的表达从胚胎第15天到出生后第40天增加。这是关于大鼠肺中存在PSP及其参与细胞生长和分化调节的首次报道。
