Narita Masaru, Chiba Kazuyuki, Nishizawa Hiroshi, Ishii Hidenori, Huang Chieh-Chen, Kawabata Zen'ichiro, Silver Simon, Endo Ginro
Laboratory of Environmental Biotechnology, Faculty of Engineering, Tohoku Gakuin University, Tagajo, 985-8537, Japan.
FEMS Microbiol Lett. 2003 Jun 6;223(1):73-82. doi: 10.1016/S0378-1097(03)00325-2.
Thirty mercury-resistant (Hg R) Bacillus strains were isolated from mercury-polluted sediment of Minamata Bay, Japan. Mercury resistance phenotypes were classified into broad-spectrum (resistant to inorganic Hg(2+) and organomercurials) and narrow-spectrum (resistant to inorganic Hg(2+) and sensitive to organomercurials) groups. Polymerase chain reaction (PCR) product sizes and the restriction nuclease site maps of mer operon regions from all broad-spectrum Hg R Bacillus were identical to that of Bacillus megaterium MB1. On the other hand, the PCR products of the targeted merP (extracellular mercury-binding protein gene) and merA (intracellular mercury reductase protein gene) regions from the narrow-spectrum Hg R Bacillus were generally smaller than those of the B. megaterium MB1 mer determinant. Diversity of gene structure configurations was also observed by restriction fragment length polymorphism (RFLP) profiles of the merA PCR products from the narrow-spectrum Hg R Bacillus. The genetic diversity of narrow-spectrum mer operons was greater than that of broad-spectrum ones.
从日本水俣湾汞污染沉积物中分离出30株耐汞（Hg R）芽孢杆菌菌株。耐汞表型分为广谱（对无机Hg(2+)和有机汞化合物耐药）和窄谱（对无机Hg(2+)耐药且对有机汞化合物敏感）两组。所有广谱Hg R芽孢杆菌的mer操纵子区域的聚合酶链反应（PCR）产物大小和限制性核酸酶位点图谱与巨大芽孢杆菌MB1的相同。另一方面，窄谱Hg R芽孢杆菌的目标merP（细胞外汞结合蛋白基因）和merA（细胞内汞还原酶蛋白基因）区域的PCR产物通常比巨大芽孢杆菌MB1的mer决定簇的小。通过窄谱Hg R芽孢杆菌merA PCR产物的限制性片段长度多态性（RFLP）图谱也观察到基因结构构型的多样性。窄谱mer操纵子的遗传多样性大于广谱mer操纵子的遗传多样性。
