Nakamura K, Silver S
Department of Basic Medical Sciences, National Institute of Minamata Disease, Kumamoto Prefecture, Japan.
Appl Environ Microbiol. 1994 Dec;60(12):4596-9. doi: 10.1128/aem.60.12.4596-4599.1994.
Bacillus isolates from Minamata Bay sediment were selected for the ability to volatilize mercury from a range of organomercurials (including methylmercury). Chromosomal DNA from 74 of 78 such strains hybridized with the mer mercury resistance operon DNA from marine Bacillus sp. strain RC607 (Y. Wang, M. Moore, H. S. Levinson, S. Silver, C. Walsh, and I. Mahler, J. Bacteriol. 171:83-92, 1989). The most frequent classes with regard to restriction nuclease site maps of the mer operon for the new isolates were identical to or closely related to the mer determinant of strain RC607. PCR amplification analysis with primers designed from the strain RC607 mer operon gave products of precisely the predicted size with the 74 Minamata Bay isolates.
从水俣湾沉积物中分离出的芽孢杆菌菌株，因其能够从一系列有机汞化合物（包括甲基汞）中挥发汞的能力而被挑选出来。78株此类菌株中的74株的染色体DNA与海洋芽孢杆菌菌株RC607的mer汞抗性操纵子DNA杂交（Y. Wang、M. Moore、H. S. Levinson、S. Silver、C. Walsh和I. Mahler，《细菌学杂志》171:83 - 92，1989年）。关于新分离株mer操纵子的限制性核酸酶位点图谱，最常见的类别与菌株RC607的mer决定簇相同或密切相关。用根据菌株RC607 mer操纵子设计的引物进行的PCR扩增分析，在74株水俣湾分离株中得到了精确预测大小的产物。
